您好，欢迎访问广东省农业科学院 机构知识库！
广东省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

OTP970 Is Required for RNA Editing of Chloroplast ndhB Transcripts in Arabidopsis thaliana

文献类型： 外文期刊

作者： Fu, Mei 1 ; Lin, Xiaona 2 ; Zhou, Yining 2 ; Zhang, Chunmei 1 ; Liu, Bing 1 ; Feng, Dongru 1 ; Wang, Jinfa 1 ; Wang, Hongbin 2 ; Jin, Honglei 2 ;

作者机构： 1.Sun Yat Sen Univ, Sch Life Sci, Guangzhou 510275, Peoples R China

2.Guangzhou Univ Chinese Med, Sch Pharmaceut Sci, Inst Med Plant Physiol & Ecol, Guangzhou 510006, Peoples R China

3.Guangdong Acad Agr Sci, Vegetable Res Inst, Guangzhou 510006, Peoples R China

4.Guangdong Key Lab New Technol Res Vegetables, Guangzhou 510006, Peoples R China

关键词： Arabidopsis; chloroplast; ndhB-C149; OTP970; PPR; RNA editing

期刊名称：GENES （ 影响因子：4.141； 五年影响因子：4.474 ）

ISSN：

年卷期： 2022 年 13 卷 1 期

页码：

收录情况： SCI

摘要： RNA editing is essential for compensating for defects or mutations in haploid organelle genomes and is regulated by numerous trans-factors. Pentatricopeptide repeat (PPR) proteins are the prime factors that are involved in RNA editing; however, many have not yet been identified. Here, we screened the plastid-targeted PLS-DYW subfamily of PPR proteins belonging to Arabidopsis thaliana and identified ORGANELLE TRANSCRIPT PROCESSING 970 (OTP970) as a key player in RNA editing in plastids. A loss-of-function otp970 mutant was impaired in RNA editing of ndhB transcripts at site 149 (ndhB-C149). RNA-immunoprecipitation analysis indicated that OTP970 was associated with the ndhB-C149 site. The complementation of the otp970 mutant with OTP970 lacking the DYW domain (OTP970(Delta DYW)) failed to restore the RNA editing of ndhB-C149. ndhB gene encodes the B subunit of the NADH dehydrogenase-like (NDH) complex; however, neither NDH activity and stability nor NDH-PSI supercomplex formation were affected in otp970 mutant compared to the wild type, indicating that alteration in amino acid sequence is not necessary for NdhB function. Together, these results suggest that OTP970 is involved in the RNA editing of ndhB-C149 and that the DYW domain is essential for its function.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广东省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台