OTP970 Is Required for RNA Editing of Chloroplast ndhB Transcripts in Arabidopsis thaliana
文献类型： 外文期刊
作者： Fu, Mei 1 ; Lin, Xiaona 2 ; Zhou, Yining 2 ; Zhang, Chunmei 1 ; Liu, Bing 1 ; Feng, Dongru 1 ; Wang, Jinfa 1 ; Wang, Hongbin 2 ; Jin, Honglei 2 ;
作者机构： 1.Sun Yat Sen Univ, Sch Life Sci, Guangzhou 510275, Peoples R China
2.Guangzhou Univ Chinese Med, Sch Pharmaceut Sci, Inst Med Plant Physiol & Ecol, Guangzhou 510006, Peoples R China
3.Guangdong Acad Agr Sci, Vegetable Res Inst, Guangzhou 510006, Peoples R China
4.Guangdong Key Lab New Technol Res Vegetables, Guangzhou 510006, Peoples R China
关键词： Arabidopsis; chloroplast; ndhB-C149; OTP970; PPR; RNA editing
期刊名称：GENES （ 影响因子：4.141； 五年影响因子：4.474 ）
ISSN：
年卷期： 2022 年 13 卷 1 期
页码：
收录情况： SCI
摘要： RNA editing is essential for compensating for defects or mutations in haploid organelle genomes and is regulated by numerous trans-factors. Pentatricopeptide repeat (PPR) proteins are the prime factors that are involved in RNA editing; however, many have not yet been identified. Here, we screened the plastid-targeted PLS-DYW subfamily of PPR proteins belonging to Arabidopsis thaliana and identified ORGANELLE TRANSCRIPT PROCESSING 970 (OTP970) as a key player in RNA editing in plastids. A loss-of-function otp970 mutant was impaired in RNA editing of ndhB transcripts at site 149 (ndhB-C149). RNA-immunoprecipitation analysis indicated that OTP970 was associated with the ndhB-C149 site. The complementation of the otp970 mutant with OTP970 lacking the DYW domain (OTP970(Delta DYW)) failed to restore the RNA editing of ndhB-C149. ndhB gene encodes the B subunit of the NADH dehydrogenase-like (NDH) complex; however, neither NDH activity and stability nor NDH-PSI supercomplex formation were affected in otp970 mutant compared to the wild type, indicating that alteration in amino acid sequence is not necessary for NdhB function. Together, these results suggest that OTP970 is involved in the RNA editing of ndhB-C149 and that the DYW domain is essential for its function.
- 相关文献
作者其他论文 更多>>
-
Identification and expression profiles of tubby-like proteins coding genes in Brassica rapa (B. rapa) in response to hormone and drought stress
作者：Khan, Imran;Guo, Juxian;Karamat, Umer;Li, Guihua;Fu, Mei
关键词：Genome-wide analysis;
Brassica rapa ; Tubby-like proteins; Drought; Hormones -
Adaptive responses of plants to light stress: mechanisms of photoprotection and acclimation. A review
作者：Khan, Imran;Li, Guihua;Fu, Mei;Zaman, Shah
关键词：climate change; light; reactive oxygen species; biogenesis of PSII; photosystem II repair cycle
-
Integrated omics and functional insights into BjMYB90-mediated regulation of BjGSTF12 for enhanced anthocyanin biosynthesis in mustard (Brassica juncea)
作者：Karamat, Umer;Guo, Juxian;Jiang, Shizheng;Khan, Imran;Lu, Mengting;Li, Guihua;Fu, Mei
关键词：GST;
B. juncea ; Metabolome; Transcriptome; Expression analysis; Anthocyanin transport -
BrGSTF12, an anthocyanin-related glutathione S-transferase gene, is essential for light-induced anthocyanin accumulation in zicaitai (Brassica Rapa Var. purpuraria)
作者：Fu, Mei;Li, Guihua;Lu, Mengting;Jiang, Shizheng;Karamat, Umer;Khan, Imran;Guo, Juxian;Lu, Mengting;Jiang, Shizheng
关键词：Transcriptome; RNA-seq; Anthocyanin; qRT-PCR; Zicaitai
-
Integrated metabolome and transcriptome analyses reveal the role of BoGSTF12 in anthocyanin accumulation in Chinese kale (Brassica oleracea var. alboglabra)
作者：Tang, Kang;Jiang, Shizheng;Yang, Xian;Tang, Kang;Karamat, Umer;Li, Guihua;Guo, Juxian;Jiang, Shizheng;Fu, Mei
关键词：Metabolome; RNA-seq; Anthocyanins; qRT-PCR; Brassica oleracea
-
Comparative Metabolome and Transcriptome Analyses Reveal the Regulatory Mechanism of Purple Leafstalk Production in Taro (Colocasia esculenta L. Schott)
作者：Jiang, Shizheng;Guo, Juxian;Khan, Imran;Tang, Kang;Li, Guihua;Fu, Mei;Jiang, Shizheng;Tang, Kang;Yang, Xian;Jahan, Mohammad Shah
关键词：taro leafstalk; metabolome; transcriptome; anthocyanins
-
Comprehensive, Genome-Wide Identification and Expression Analyses of Phenylalanine Ammonia-Lyase Family under Abiotic Stresses in Brassica oleracea
作者：Karamat, Umer;Guo, Juxian;Jiang, Shizheng;Khan, Imran;Lu, Mengting;Fu, Mei;Li, Guihua
关键词：phenylalanine ammonia-lyase; PAL; B. oleracea; cold; abscisic acid; abiotic stress
