Engineered Exosomes Delivering bta-miR-223 Alleviate Staphylococcus aureus-Induced Mastitis in Mice via the TLR4/NF-κB Signaling Pathway
文献类型： 外文期刊
作者： Wang, Yuxuan 1 ; Ouyang, Kun 1 ; Nie, Ying 1 ; Yang, Jingshuo 1 ; Liao, Yunxin 1 ; Chen, Xingping 2 ; Wang, Hailong 1 ; Luo, Junyi 1 ; Sun, Jiajie 1 ; Xi, Qianyun 1 ; Shen, Binglei 3 ; Chen, Ting 1 ; Zhang, Yongliang 1 ;
作者机构： 1.South China Agr Univ, Guangdong Prov Key Lab Anim Nutr Regulat, State Key Lab Livestock & Poultry Breeding, Natl Engn Res Ctr Breeding Swine Ind,Coll Anim Sci, Guangzhou 510642, Guangdong, Peoples R China
2.Jiangxi Agr Univ, Key Lab Anim Nutr Jiangxi Prov, Nanchang 330045, Jiangxi, Peoples R China
3.Heilongjiang Bayi Agr Univ, Heilongjiang Prov Key Lab Prevent & Control Bovine, Daqing 163000, Heilongjiang, Peoples R China
关键词：
bovine mastitis; engineered exosomes; bta-miR-223; nanotherapeutics;
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.2； 五年影响因子：6.4 ）
ISSN： 0021-8561
年卷期： 2025 年 73 卷 28 期
页码：
收录情况： SCI
摘要： Bovine mastitis is an inflammatory disease of the mammary gland, frequently associated with infection by Staphylococcus aureus. Effective delivery of the antiinflammatory microRNA bta-miR-223 remains a challenge. In this study, we constructed engineered exosomes loaded with bta-miR-223 and demonstrated their antiinflammatory effects both in vitro (Mac-T cells) and in vivo (mice). Overexpression of bta-miR-223 reduced the expression of IL-6 and IL-1 beta in a lipoteichoic acid-induced Mac-T cell inflammation model. Following tail vein injection in lactating mice, the engineered exosomes accumulated in the mammary gland, alleviated S. aureus-induced inflammation, and increased the expression of barrier-related proteins ZO-1, claudin-1, and occludin. Mechanistically, bta-miR-223 inhibited RHOB expression and modulated the TLR4/NF-kappa B pathway. These results demonstrate that exosome-mediated delivery of bta-miR-223 effectively alleviates mammary inflammation, providing a novel strategy for nucleic acid nanotherapy.
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