A Deacetylase CqSIRT1 Promotes WSSV Infection by Binding to Viral Envelope Proteins in Cherax quadricarinatus
文献类型： 外文期刊
作者： Zheng, Shucheng 1 ; Meng, Fanjuan 1 ; Li, Dongli 1 ; Liu, Lingke 1 ; Ge, Di 1 ; Wang, Qing 3 ; Liu, Haipeng 1 ;
作者机构： 1.Xiamen Univ, State Key Lab Marine Environm Sci, State Prov Joint Engn Lab Marine Bioprod & Techno, Coll Ocean Earth Sci, Xiamen 361102, Peoples R China
2.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266100, Peoples R China
3.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Fishery Drug Dev,Minist Agr, Key Lab Aqut Anim Immune Technol Guangdong Prov, Guangzhou 510380, Peoples R China
关键词： SIRT1; lysine deacetylases; white spot syndrome virus; envelope proteins; Cherax quadricarinatus
期刊名称：VIRUSES-BASEL （ 影响因子：5.818； 五年影响因子：5.811 ）
ISSN：
年卷期： 2022 年 14 卷 8 期
页码：
收录情况： SCI
摘要： Sirtuin 1 (SIRT1), a member of the class III lysine deacetylases, exhibits powerful functional diversity in physiological processes and disease occurrences. However, the potential molecular mechanism underlying the role of SIRT1 during viral infection in crustaceans is poorly understood. Herein, SIRT1 was functionally characterized from the red claw crayfish Cherax quadricarinatus, which possesses typically conserved deacetylase domains and strong evolutionary relationships across various species. Moreover, gene knockdown of CqSIRT1 in crayfish haematopoietic tissue (Hpt) cell culture inhibited white spot syndrome virus (WSSV) late envelope gene vp28 transcription. In contrast, enhancement of deacetylase activity using a pharmacological activator promoted the replication of WSSV. Mechanically, CqSIRT1 was co-localized with viral envelope protein VP28 in the nuclei of Hpt cells and directly bound to VP28 with protein pulldown and co-immunoprecipitation assays. Furthermore, CqSIRT1 also interacted with another two viral envelope proteins, VP24 and VP26. To the best of our knowledge, this is the first report that WSSV structural proteins are linked to lysine deacetylases, providing a better understanding of the role of CqSIRT1 during WSSV infection and novel insights into the basic mechanism underlying the function of lysine deacetylases in crustaceans.
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