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Cumulus cells orchestrate oocyte maturation via actin polymerization and vitamin B6 metabolism regulation: Highlights from multi-omics analysis in sheep vitrified cumulus-oocyte complex

文献类型： 外文期刊

作者： Bai, Jiachen 1 ; Hai, Guiping 3 ; Liu, Yucheng 2 ; Li, Jun 4 ; Liu, Aiju 1 ; Zhang, Zhenliang 2 ; Wang, Longfei 1 ; Wang, Jingjing 2 ; Guo, Yanhua 2 ; Wan, Pengcheng 2 ; Fu, Xiangwei 1 ;

作者机构： 1.China Agr Univ, Coll Anim Sci & Technol, Key Lab Anim Genet Breeding & Reprod MARA, Natl Engn Lab Anim Breeding,State Key Lab Anim Bio, Beijing 100193, Peoples R China

2.Inst Anim Husb & Vet Sci, Xinjiang Acad Agr & Reclamat Sci, State Key Lab Sheep Genet Improvement & Hlth Breed, Shihezi 832000, Peoples R China

3.Jilin Agr Univ, Minist Educ, Key Lab Anim Prod Prod Qual & Secur, Changchun 130118, Jilin, Peoples R China

4.Hebei Med Univ, Hosp 1, Reprod Med Ctr, Dept Reprod Med, Shijiazhuang 050000, Peoples R China

关键词： Cumulus-oocytes complexes; Vitrification; Actin polymerization; Vitamin B 6; Multi-omics; Sheep

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2025 年 320 卷

页码：

收录情况： SCI

摘要： It is well known that cumulus cells play a crucial role in meiotic resumption and oocyte maturation. However, the role of cumulus cells in oocytes development under vitrification stress remains elusive. Therefore, we sought to delineate the molecular mechanism underlying the interaction of cumulus cells and oocytes developmental potential under stress conditions by integration analysis (Pathway co-enrichment, Protein network clustering, and Pearson correlation analysis) based on 4D Label free proteome, Ultra-Low RNA-seq and untargeted metabolome techniques. "Regulation of actin cytoskeleton" and "vitamin B6 metabolism" were identified as the most enriched and impacted pathways. Moreover, our results showed that the absence of cumulus cells significantly reduced oocyte survival, maturation, and normal F-actin distribution rate after IVM. Notably, Narciclasine (an agent functions in promoting actin polymerization) significantly improved the polar body extrusion (PBE) rate and reduced cortical tension defects rate of vitrified oocytes after in vitro maturation (IVM). What's more, we discovered that vitrification significantly reduced mitochondrial membrane potential (MMP) level in cumulus cells and ATP content in oocytes. Whereas, Pyridoxal 5 '-phosphate monohydrate (the active form of vitamin B6) improved ATP level and cytoplasmic maturation of vitrified oocytes after IVM. In conclusion, our results demonstrated the value of multi-omics approach in deciphering the role of cumulus cells during ovine oocyte growth under stress conditions, and revealed that promoting actin polymerization and vitamin B6 metabolism contributes to the improvement of vitrified ovine oocytes quality.

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