Ultrasonic-assisted extraction and high-speed counter-current chromatography purification of zeaxanthin dipalmitate from the fruits of Lycium barbarum L.
文献类型： 外文期刊
作者： Kan, Xuhui 1 ; Yan, Yamei 2 ; Ran, Linwu 3 ; Lu, Lu 2 ; Mi, Jia 2 ; Zhang, Zhijuan 2 ; Li, Xiaoying 2 ; Zeng, Xiaoxiong 1 ; Cao, Youlong 2 ;
作者机构： 1.Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Jiangsu, Peoples R China
2.Natl Wolfberry Engn Res Ctr, Yinchuan 750002, Ningxia, Peoples R China
3.Ningxia Med Univ, Lab Anim Ctr, Yinchuan 750004, Ningxia, Peoples R China
关键词： Lycium barbarum L.; Carotenoids; Zeaxanthin dipalmitate; HPLC analysis; Ultrasonic-assisted extraction; High-speed counter-current chromatography
期刊名称：FOOD CHEMISTRY （ 影响因子：9.231； 五年影响因子：8.795 ）
ISSN： 0308-8146
年卷期： 2020 年 310 卷
页码：
收录情况： SCI
摘要： Zeaxanthin dipalmitate (ZDP) is a major non-saponified carotenoid in fully ripe fruits of Lycium barbarum L. In the present study, response surface methodology was used to optimize the ultrasonic-assisted extraction (UAE) conditions of carotenoids from the fruits of L. barbarum, and the optimal extraction conditions were determined as follows: ultrasonic power of 360 W, ultrasonic time of 40 min and the ratio of extraction solvent to sample of 30 mL/g. An actual value of ZDP content of 5.40 mg/g and short extraction time indicated the efficiency of UAE. Furthermore, a promising high-speed counter-current chromatography (HSCCC) method was established for the purification of ZDP from the fruits of L. barbarum. With a developed two-phase solvent system composed of nhexane/dichloromethane/acetonitrile (10/3/7, v/v/v), ZDP with a purity of higher than 95% was successfully isolated from the crude extract. This is the first report on the purification of ZDP by using HSCCC.
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