Isolation, sequencing of the HvnHID gene and its role in the purple-grain colour development in Tibetan hulless barley
文献类型： 外文期刊
作者： Yao, Xiaohua 1 ; Su, Leping 1 ; Yao, Youhua 1 ; An, Likun 1 ; Bai, Yixiong 1 ; Li, Xin 1 ; Wu, Kunlun 1 ;
作者机构： 1.Qinghai Univ, Xining, Peoples R China
2.Qinghai Acad Agr & Forestry Sci, Xining, Peoples R China
3.Qinghai Key Lab Hulless Barley Genet & Breeding, Xining, Peoples R China
4.Qinghai Subctr Natl Hulless Barley Improvement, Xining, Peoples R China
5.Yanan Inst Agr Sci, Yanan, Peoples R China
关键词： 2-hydroxyisoflavanone dehydratase; anthocyanidin; isoflavones; purple grain
期刊名称：CZECH JOURNAL OF GENETICS AND PLANT BREEDING （ 影响因子：0.865； 五年影响因子：1.017 ）
ISSN： 1212-1975
年卷期：
页码：
收录情况： SCI
摘要： 2-hydroxyisoflavanone dehydratase (HID) plays an important role in isoflavone biosynthesis. In this study, HID was isolated from the seeds of the purple-grained Tibetan hulless barley variety Nerumuzha and the white-grained variety Kunlun 10. The HvnHID gene includes the 981 bp open reading frame and encodes a protein of 327 amino acids. It has a typical Abhydrolase_3 domain (78-306) and belongs to the carboxylesterase (CXE) family of the Abhydrolase_3 (alpha/P hydrolase) superfamily. There are eight nucleotide differences in the HvnHID coding sequence and two amino acid differences (one in the Abhydrolase_3 domain) between Nerumuzha and Kunlun 10. The HvnHID of hulless barley has the closest relationship with the HID in Hordeum vulgare, and the most distant relationship in Panicum hallii. At the early-mid stage of the seed colour development, the HvnHID expression levels in the purple and black seeds were significantly higher than in the white and blue ones (P < 0.01). During the seed colour development of purple-grained hulless barley, the expression of the key genes (HvnF3'H, HvnDRF, HvnANT1, and HvnGT) in the anthocyanidin biosynthetic pathway increased significantly, while the HvnHID expression decreased significantly (P < 0.01). Thus, it is likely that HvnHID negatively regulates the anthocyanidin biosynthesis. This result provides an important basis for further study of the biological functions of HvnHID in the anthocyanidin biosynthetic pathway.
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