Construction of SNP Fingerprinting and Genetic Diversity Analysis of Eggplant Based on KASP Technology
文献类型： 外文期刊
作者： Wang, Wuhong 1 ; Pang, Hongtao 1 ; Hu, Na 1 ; Hu, Haijiao 1 ; Hu, Tianhua 1 ; Yan, Yaqin 1 ; Wang, Jinglei 1 ; Ai, Jiaqi 1 ; Bao, Chonglai 1 ; Wei, Qingzhen 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, Inst Vegetable, Hangzhou 310021, Peoples R China
关键词： eggplant; KASP; DNA fingerprinting; genetic diversity; population structure
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）
ISSN： 1661-6596
年卷期： 2025 年 26 卷 11 期
页码：
收录情况： SCI
摘要： Eggplant (Solanum melongena) is a significant vegetable in the Solanaceae family. Significant progress has been made in genetic diversity analysis and fingerprinting construction for crops such as tomatoes and peppers within the same family, but research on eggplants in these aspects remains relatively limited. Current germplasm identification using fingerprinting primarily relies on traditional SSR markers, which suffer from limited polymorphism and labor-intensive workflows. This study aimed to identify high-quality single nucleotide polymorphisms (SNPs), develop reliable Kompetitive Allele-Specific PCR (KASP) markers for eggplant genotyping, and then conduct fingerprint construction and genetic diversity analysis. The ultimate goals were to achieve a precise identification of eggplant varieties and deeply explore the genetic background and evolutionary patterns of eggplant germplasm. In this study, 49 representative eggplant accessions were re-sequenced. After data quality control, sequence alignment, and multiple rounds of screening, 224 high-quality SNPs were identified. Based on these SNPs, 96 SNPs were selected to develop KASP markers. These markers can provide abundant genetic markers for eggplant genetic research, which are used to deeply explore the genetic background and conduct genetic diversity analysis. After multiple rounds of rigorous verification, 32 core candidate markers were finally screened out. The average polymorphic information content (PIC) and gene diversity (GD) values were 0.36 and 0.46, respectively. Phylogenetic tree, population structure, and principal component analyses divided the 280 eggplant accessions into eight distinct groups. Through the analysis of minimal core markers and core germplasm, 23 core SNP markers and a subset of 56 core germplasm accessions were identified, leading to the establishment of a comprehensive fingerprinting system for all 280 accessions. Our findings provide a foundational genetic resource for eggplant germplasm identification and offer significant support for future breeding efforts.
- 相关文献
作者其他论文 更多>>
-
Genome-Wide Identification and Expression Analysis of Eggplant Reveals the Key MYB Transcription Factor Involved in Anthocyanin Synthesis
作者：Ai, Jiaqi;Hu, Na;Ai, Jiaqi;Wang, Wuhong;Hu, Tianhua;Hu, Haijiao;Yan, Yaqin;Wang, Jinglei;Wang, Yunzhu;Hu, Na;Pang, Hongtao;Bao, Chonglai;Wei, Qingzhen
关键词：eggplant; MYB gene family; genome-wide identification; anthocyanin; RNA-seq
-
Genome-wide identification of histone acetyltransferase genes in Solanum melongena reveals two critical regulators of Botrytis cinerea resistance
作者：Yan, Yaqin;Wang, Pengfei;Wang, Wuhong;Hu, Haijiao;Hu, Tianhua;Wei, Qingzhen;Bao, Chonglai
关键词：Eggplant; Bioinformatics; Histone acetyltransferase; Botrytis cinerea; Disease resistance
-
Identification of Quantitative Trait Loci and Candidate Genes Controlling Seed Dormancy in Eggplant (Solanum melongena L.)
作者：Ai, Jiaqi;Wang, Wuhong;Hu, Tianhua;Hu, Haijiao;Wang, Jinglei;Yan, Yaqin;Pang, Hongtao;Bao, Chonglai;Wei, Qingzhen;Ai, Jiaqi;Pang, Hongtao;Wang, Yong
关键词：Solanum melongena L.; seed dormancy; QTL-Seq; candidate gene
-
Fine Mapping of Candidate Gene Controlling Anthocyanin Biosynthesis for Purple Peel in Solanum melongena L.
作者：Xiao, Kai;Tan, Feng;Zhang, Aidong;Zhou, Yaru;Zhu, Weimin;Zha, Dingshi;Wu, Xuexia;Bao, Chonglai
关键词：anthocyanin; eggplant; peel color; candidate gene
-
Construction of SNP fingerprints and genetic diversity analysis of radish (Raphanus sativus L.)
作者：Xing, Xiaolin;Gan, Defang;Xing, Xiaolin;Hu, Tianhua;Wang, Wuhong;Hu, Haijiao;Wei, Qingzhen;Yan, Yaqin;Bao, Chonglai;Wang, Jinglei;Wang, Yikui;Li, Yan
关键词：radish; SNP; KASP; fingerprint; genetic diversity
-
Comprehensive Genomic and Proteomic Analysis Identifies Effectors of Fusarium oxysporum f. sp. melongenae
作者：Wu, Jiayelu;Wang, Pengfei;Wang, Wuhong;Hu, Haijiao;Wei, Qingzhen;Bao, Chonglai;Yan, Yaqin
关键词：effector proteins;
F. oxysporum f. sp.melongenae ; bioinformatic prediction; LC-MS -
Metabolic and Transcriptomic Analyses Reveal Different Metabolite Biosynthesis Profiles between Purple and Green Pak Choi
作者：Wang, Jinglei;Hu, Tianhua;Wang, Yidi;Wang, Wuhong;Hu, Haijiao;Wei, Qingzhen;Yan, Yaqin;Bao, Chonglai;Wang, Yidi
关键词：pak choi; transcriptomes; metabolism; anthocyanin; MYB2
