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Identification of Quantitative Trait Loci and Candidate Genes Controlling Seed Dormancy in Eggplant (Solanum melongena L.)

文献类型： 外文期刊

作者： Ai, Jiaqi 1 ; Wang, Wuhong 1 ; Hu, Tianhua 1 ; Hu, Haijiao 1 ; Wang, Jinglei 1 ; Yan, Yaqin 1 ; Pang, Hongtao 1 ; Wang, Yong 3 ; Bao, Chonglai 1 ; Wei, Qingzhen 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Hangzhou 310021, Peoples R China

2.Zhejiang Agr & Forestry Univ, Coll Hort Sci, Hangzhou 310021, Peoples R China

3.Zhumadian Acad Agr Sci, Zhumadian 463000, Peoples R China

关键词： Solanum melongena L.; seed dormancy; QTL-Seq; candidate gene

期刊名称：GENES （ 影响因子：3.5； 五年影响因子：3.9 ）

ISSN：

年卷期： 2024 年 15 卷 4 期

页码：

收录情况： SCI

摘要： Seed dormancy is a life adaptation trait exhibited by plants in response to environmental changes during their growth and development. The dormancy of commercial seeds is the key factor affecting seed quality. Eggplant seed dormancy is controlled by quantitative trait loci (QTLs), but reliable QTLs related to eggplant dormancy are still lacking. In this study, F-2 populations obtained through the hybridization of paternally inbred lines with significant differences in dormancy were used to detect regulatory sites of dormancy in eggplant seeds. Three QTLs (dr1.1, dr2.1, and dr6.1) related to seed dormancy were detected on three chromosomes of eggplant using the QTL-Seq technique. By combining nonsynonymous sites within the candidate regions and gene functional annotation analysis, nine candidate genes were selected from three QTL candidate regions. According to the germination results on the eighth day, the male parent was not dormant, but the female parent was dormant. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to verify the expression of nine candidate genes, and the Smechr0201082 gene showed roughly the same trend as that in the phenotypic data. We proposed Smechr0201082 as the potential key gene involved in regulating the dormancy of eggplant seeds. The results of seed experiments with different concentrations of gibberellin A(3) (GA(3)) showed that, within a certain range, the higher the gibberellin concentration, the earlier the emergence and the higher the germination rate. However, higher concentrations of GA(3) may have potential effects on eggplant seedlings. We suggest the use of GA(3) at a concentration of 200-250 mgL-1 to treat dormant seeds. This study provides a foundation for the further exploration of genes related to the regulation of seed dormancy and the elucidation of the molecular mechanism of eggplant seed dormancy and germination.

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