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Rice Brittle Culm19 Encoding Cellulose Synthase Subunit CESA4 Causes Dominant Brittle Phenotype But has No Distinct Influence on Growth and Grain Yield

文献类型： 外文期刊

作者： Ma, Xiaozhi 1 ; Li, Chunmei 1 ; Huang, Rui 1 ; Zhang, Kuan 1 ; Wang, Qian 1 ; Fu, Chongyun 2 ; Liu, Wuge 2 ; Sun, Changhu 1 ;

作者机构： 1.Sichuan Agr Univ, Rice Res Inst, State Key Lab Crop Gene Explorat & Utilizat South, Chengdu 611130, Peoples R China

2.Guangdong Acad Agr Sci, Rice Res Inst, Guangdong Prov Key Lab New Technol Rice Breeding, Guangzhou 510642, Peoples R China

3.Zhongkai Univ Agr & Engn, Coll Agr & Biol, Guangzhou Key Lab Res & Dev Crop Germplasm Resour, Guangzhou 510225, Peoples R China

4.Shanxi Agr Univ, Ctr Agr Genet Resources Res, Taiyuan 030031, Peoples R China

关键词： Rice; Dominant mutation; Brittle culm; Cellulose synthase; Secondary cell wall

期刊名称：RICE （ 影响因子：4.783； 五年影响因子：5.23 ）

ISSN： 1939-8425

年卷期： 2021 年 14 卷 1 期

页码：

收录情况： SCI

摘要： Background Mechanical strength is a crucial agronomic trait in rice (Oryza sativa), and brittle mutants are thought suitable materials to investigate the mechanism of cell wall formation. So far, almost all brittle mutants are recessive, and most of them are defected in multiple morphologies and/or grain yield, limiting their application in hybrid breeding and in rice straw recycling. Results We identified a semi-dominant brittle mutant Brittle culm19 (Bc19) isolated from the japonica variety Nipponbare through chemical mutagenesis. The mutant showed the same apparent morphologies and grain yield to the wild type plant except for its weak mechanical strength. Its development of secondary cell wall in sclerenchyma cells was affected, along with reduced contents of cellulose, hemicellulose, lignin and sugars in culms and leaves. Positional cloning suggested that the Bc19 gene was allelic to OsCESA4, encoding one of the cellulose synthase A (CESA) catalytic subunits. In this mutant, a C-to-T substitution occurred in the coding sequence of BC19, causing the P507S missense mutation in its encoded product, which was located in the second cytoplasmic region of the OsCESA4 protein. Furthermore, introducing mutant gene Bc19 into the wild-type plant resulted in brittle plants, confirming that the P507S point mutation in OsCESA4 protein was responsible for the semi-dominant brittle phenotype of Bc19 mutant. Reverse correlation was revealed between cellulose contents and expression levels of mutant gene Bc19 among the homozygous mutant, the hybrid F-1 plant, and the Bc19 overexpression transgenic plants, implying that gene Bc19 might affect cellulose synthesis in a dosage-dependent manner. Conclusions Bc19, a semi-dominant brittle mutant allele of gene OsCESA4, was identified using map-based cloning approach. The mutated protein of Bc19 possessing the P507S missense mutation behaved in a dosage-dependent semi-dominant manner. Unique brittle effect on phenotype and semi-dominant genetic quality of gene Bc19 indicated its potential application in grain-straw dual-purpose hybrid rice breeding.

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