In-depth interpretation of aptamer-based sensing on electrode: Dual-mode electrochemical-photoelectrochemical sensor for the ratiometric detection of patulin
文献类型： 外文期刊
作者： Liu, Shuda 1 ; Meng, Shuyun 1 ; Wang, Meng 2 ; Li, Wenjia 1 ; Dong, Na 1 ; Liu, Dong 1 ; Li, Yuye 1 ; You, Tianyan 1 ;
作者机构： 1.Jiangsu Univ, Sch Agr Engn, Key Lab Modern Agr Equipment & Technol, Minist Educ, Zhenjiang 212013, Jiangsu, Peoples R China
2.Beijing Acad Agr & Forestry Sci, Inst Qual Stand & Testing Technol, Beijing 100097, Peoples R China
关键词： Patulin; Aptamer; Conformation change; Dual -mode detection; Ratiometric aptasensor
期刊名称：FOOD CHEMISTRY （ 影响因子：8.8； 五年影响因子：8.6 ）
ISSN： 0308-8146
年卷期： 2023 年 410 卷
页码：
收录情况： SCI
摘要： Electrochemical aptasensors have been extensively used to quantify food contaminants (e.g., mycotoxin) by using high-affinity aptamer for target recognition. Yet, analytical performance of aptasensors using different aptamers can be varied for the same target. Here, four aptamers with different sequences (i.e., A22, A34, A42, and A45) of patulin (PAT) were selected to estimate sensing behaviors at electrodes with electrochemical (EC) and photoelectrochemical (PEC) assays. Synergistic effect of steric hindrance and electron transfer distance was found to significantly affect EC and PEC response for PAT at aptasensors fabricated with A22, A34, A42, or A45. Eventually, A22 emerged to be the optimal aptamer for aptasensing, despite the highest affinity of A42 to PAT. The A22-based EC-PEC dual-mode ratiometric aptasensor offered a linear range of 50 fg mL-1 - 500 ng mL-1 with a detection limit of 30 fg mL-1 for PAT, and it was applied to apple product (i.e., juice, puree) analysis.
- 相关文献
作者其他论文 更多>>
-
Preparation of Hybrid Magnetic Nanoparticles for Sensitive and Rapid Detection of Phorate Residue in Celery Using SERS Immunochromatography Assay
作者：Li, Xiangyang;Qian, Hean;Qian, Hean;Tao, Jin;Cao, Mingshuo;Wang, Meng;Zhai, Wenlei
关键词：surface-enhanced Raman spectroscopy; immunochromatography assay; rapid detection; organophosphorus pesticide; phorate; magnetic nanoparticles; immunochromatographic test strip
-
Constructing graphene oxide/Au nanoparticle cellulose membranes for SERS detection of mixed pesticide residues in edible chrysanthemum
作者：Zhao, Zhilei;Cao, Mingshuo;Cao, Mingshuo;Wei, Dizhe;Wang, Meng;Zhai, Wenlei;Li, Xiangyang
关键词：
-
Regulation of nitrogen utilization and mycotoxin biosynthesis by the GATA transcription factor AaAreA in Alternaria alternata
作者：Wang, Liuqing;Wang, Meng;Wang, Cheng;Xu, Liang
关键词：Alternaria alternata; Alternariol; Mycotoxin; Nitrogen regulation; GATA transcription factor
-
Antifungal mechanism of protocatechuic acid methyl ester against Botrytis cinerea in postharvest strawberry fruit
作者：Yuan, Shuzhi;Wang, Baogang;Sun, Minmin;Wang, Xuanqi;Li, Xiaofei;Yang, Na;Xu, Xiaodi;Zheng, Shufang;Wang, Qing;Wang, Meng
关键词：Protocatechuic acid methyl ester; Botrytis cinerea; Strawberries; Antifungal mechanism
-
Mechanism of improving the quality of table grapes (Vitis. spp.) by exogenous eugenol based on transcriptome
作者：Jiang, Nan;Wang, Liuqing;Gao, Yuan;Li, Qianqian;Jiang, Dongmei;Wang, Meng;Jiang, Nan;Yao, Weirong;Jiang, Nan;Yao, Weirong
关键词：Eugenol; grape; postharvest quality; transcriptome
-
Geographical Indication Characteristics of Aroma and Phenolic Acids of the Changping Strawberry
作者：Wu, Linxia;Wang, Xinlu;Wang, Meng;Hao, Jianqiang;Zhu, Ning
关键词：aroma; characteristic markers; geographical indication; phenolicacids; strawberry
-
Advances in understanding grapevine downy mildew: From pathogen infection to disease management
作者：Peng, Junbo;Wang, Xuncheng;Wang, Hui;Li, Xinghong;Zhang, Qi;Wang, Meng;Yan, Jiye
关键词：effector biology; grapevine; Plasmopara viticola; sustainable control
