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Development and characterization of 37 SNP markers for the largemouth bass (Micropterus salmoides) by using PCR-RFLP method

文献类型： 外文期刊

作者： Jiang, Zhou 1 ; Cui, Jiao 1 ; Shao, Jiaqi 1 ; Dong, Chuanju 1 ; Du, Jinxing 2 ; Shen, Yubang 3 ; Li, Shengjie 1 ; Zhang, 1 ;

作者机构： 1.Henan Normal Univ, Coll Fisheries, Xinxiang 453007, Henan, Peoples R China

2.Chinese Acad Fisheries Sci, Pearl River Fisheries Res Inst, Key Lab Trop & Subtrop Fishery Resource Applicat, China Minist Agr, Guangzhou 510380, Peoples R China

3.Shanghai Ocean Univ, Key Lab Freshwater Aquat Genet Resources, Minist Agr & Rural Affairs, Shanghai 201306, Peoples R China

关键词： Micropterus salmoides; SNP; PCR-RFLP; Conservation genetics

期刊名称：CONSERVATION GENETICS RESOURCES （ 影响因子：0.973； 五年影响因子：0.946 ）

ISSN： 1877-7252

年卷期： 2021 年 13 卷 4 期

页码：

收录情况： SCI

摘要： Largemouth bass (Micropterus salmoides) is an economically important species in China. Contrary to its rapidly increasing yield during the last decades, the domestic genetic diversity of largemouth bass has gradually declined. In this study, 37 single nucleotide polymorphism (SNP) markers were developed based on genotyping-by-sequencing data and characterized by genotyping 32 individuals using the PCR-restriction fragment length polymorphism method. The effective number of alleles (Ne), observed heterozygosity (Ho), expected heterozygosity (He), and polymorphic information content (PIC) of these SNPs ranged from 1.168 to 1.998, 0.156 to 0.844, 0.146 to 0.507, and 0.134 to 0.375, respectively. Totally, five loci deviated significantly from Hardy-Weinberg equilibrium (p < 0.05), while there existed no linkage disequilibrium at all loci. These novel polymorphic markers will lay the foundation for future rationally excavation and utilization of germplasm resources in M. salmoides.

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