文献类型： 外文期刊

作者： Hsu, Pei-Chun (Lisa) ¹ ; Di, Hong J. ¹ ; Cameron, Keith ¹ ; Podolyan, Andriy ¹ ; Chau, Henry ¹ ; Luo, Jiafa ² ; Miller, Blair ³ ; Carrick, Sam ⁴ ; Johnstone, Paul ⁵ ; Ferguson, Scott ⁶ ; Wei, Wenhua ⁷ ; Shen, Jupei ⁸ ; Zhang, Limei ⁹ ; Liu, Hongbin ¹⁰ ; Zhao, Tongke ¹¹ ; Wei, Wenxue ¹² ; Ding, Weixin ¹³ ; Pan, Hong ¹⁴ ; Liu, Yimeng ¹⁵ ; Li, Bowen ¹⁶ ;

作者机构： 1.Lincoln Univ, Ctr Soil & Environm Res, Lincoln, New Zealand

2.AgResearch, Hamilton, New Zealand

3.Lincoln Univ, Lincoln Agritech Ltd, Lincoln, New Zealand

4.Manaaki Whenua Landcare Res, Lincoln, New Zealand

5.New Zealand Inst Plant & Food Res, Havelock North, New Zealand

6.Univ Otago, Dept Microbiol, Dunedin, New Zealand

7.Univ Otago, Dept Biochem, Dunedin, New Zealand

8.Fujian Normal Univ, Fuzhou, Peoples R China

9.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Beijing, Peoples R China

10.Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Beijing, Peoples R China

11.Beijing Acad Agr & Forestry Sci, Beijing, Peoples R China

12.Chinese Acad Sci, Inst Subtrop Agr Ecol, Changsha, Peoples R China

13.Chinese Acad Sci, Inst Soil Sci, Nanjing, Peoples R China

14.Shandong Agr Univ, Coll Nat Resources & Environm, Tai An, Peoples R China

15.Beijing Normal Univ, Ctr Innovat & Dev, Zhuhai, Peoples R China

16.Hebei Agr Univ, Coll Nat Resources & Environm, Baoding, Peoples R China

关键词： comammox Nitrospira spp; ammonia-oxidizing bacteria; nitrification; nitrite oxidation; soil microbial ecology; nitrogen cycling

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：6.064； 五年影响因子：6.843 ）

ISSN：

年卷期： 2022 年 13 卷

页码：

收录情况： SCI

摘要： The recent discovery of comammox Nitrospira, a complete ammonia oxidizer, capable of completing the nitrification on their own has presented tremendous challenges to our understanding of the nitrification process. There are two divergent clades of comammox Nitrospira, Clade A and B. However, their population abundance, community structure and role in ammonia and nitrite oxidation are poorly understood. We conducted a 94-day microcosm study using a grazed dairy pasture soil amended with urea fertilizers, synthetic cow urine, and the nitrification inhibitor, dicyandiamide (DCD), to investigate the growth and community structure of comammox Nitrospira spp. We discovered that comammox Nitrospira Clade B was two orders of magnitude more abundant than Clade A in this fertile dairy pasture soil and the most abundant subcluster was a distinctive phylogenetic uncultured subcluster Clade B2. We found that comammox Nitrospira Clade B might not play a major role in nitrite oxidation compared to the role of canonical Nitrospira nitrite-oxidizers, however, comammox Nitrospira Clade B is active in nitrification and the growth of comammox Nitrospira Clade B was inhibited by a high ammonium concentration (700 kg synthetic urine-N ha(-1)) and the nitrification inhibitor DCD. We concluded that comammox Nitrospira Clade B: (1) was the most abundant comammox in the dairy pasture soil; (2) had a low tolerance to ammonium and can be inhibited by DCD; and (3) was not the dominant nitrite-oxidizer in the soil. This is the first study discovering a new subcluster of comammox Nitrospira Clade B2 from an agricultural soil.