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The dynamics of N6-methyladenine RNA modification in resistant and susceptible rice varieties responding to rice stem borer damage

文献类型： 外文期刊

作者： Li, Shuai 1 ; Tan, Xin-Yang 2 ; He, Zhen 3 ; Shen, Chen 2 ; Li, Ya-Li 4 ; Qin, Lang 3 ; Zhao, Chun-Qing 2 ; Luo, Guang-Hua 1 ; Fang, Ji-Chao 1 ; Ji, Rui 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Plant Protect, Jiangsu Key Lab Food & Safety, State Key Lab Cultivat Base,Minist Sci & Technol, Nanjing 210014, Peoples R China

2.Nanjing Agr Univ, Coll Plant Protect, Nanjing, Peoples R China

3.Yangzhou Univ, Sch Plant Protect, Yangzhou, Jiangsu, Peoples R China

4.Wuhan Benagen Technol Co Ltd, Wuhan, Peoples R China

5.Huaiyin Normal Univ, Jiangsu Collaborat Innovat Ctr Reg Modern Agr & En, Huaian, Jiangsu, Peoples R China

6.Anhui Normal Univ, Sch Life Sci, Key Lab Conservat & Use Important Biol Resources A, Wuhu, Anhui, Peoples R China

关键词： crop variety-specific m(6)A; N-6-methyladenosine; plant-insect interactions; rice; stem borer

期刊名称：INSECT SCIENCE （ 影响因子：4.0； 五年影响因子：3.6 ）

ISSN： 1672-9609

年卷期： 2024 年

页码：

收录情况： SCI

摘要： N-6-methyladenosine (m(6)A) is the most prevalent modification in cellular RNA which orchestrates diverse physiological and pathological processes during stress response. However, the differential m(6)A modifications that cope with herbivore stress in resistant and susceptible crop varieties remain unclear. Here, we found that rice stem borer (RSB) larvae grew better on indica rice (e.g., MH63, IR64, Nanjing 11) than on japonica rice varieties (e.g., Nipponbare, Zhonghua 11, Xiushui 11). Then, transcriptome-wide m(6)A profiling of representative resistant (Nipponbare) and susceptible (MH63) rice varieties were performed using a nanopore direct RNA sequencing approach, to reveal variety-specific m(6)A modifications against RSB. Upon RSB infestation, m(6)A methylation occurred in actively expressed genes in Nipponbare and MH63, but the number of methylation sites decreased across rice chromosomes. Integrative analysis showed that m(6)A methylation levels were closely associated with transcriptional regulation. Genes involved in herbivorous resistance related to mitogen-activated protein kinase, jasmonic acid (JA), and terpenoid biosynthesis pathways, as well as JA-mediated trypsin protease inhibitors, were heavily methylated by m(6)A, and their expression was more pronounced in RSB-infested Nipponbare than in RSB-infested MH63, which may have contributed to RSB resistance in Nipponbare. Therefore, dynamics of m(6)A modifications act as the main regulatory strategy for expression of genes involved in plant-insect interactions, which is attributed to differential responses of resistant and susceptible rice varieties to RSB infestation. These findings could contribute to developing molecular breeding strategies for controlling herbivorous pests.

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