文献类型： 外文期刊

作者： Meng, Lin ¹ ; Zhou, Huimin ¹ ; Tan, Lisha ¹ ; Li, Qingyun ² ; Hou, Yujun ¹ ; Li, Wenjuan ¹ ; Kafle, Subash ¹ ; Liang, Ju ⁴ ; Aryal, Rishi ⁵ ; Liang, Zhenchang ⁶ ; Xin, Haiping ¹ ;

作者机构： 1.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty Ag, 201 Jiufeng 1st Rd,Donghu New Technol Dev Zone, Wuhan 430074, Peoples R China

2.Univ Chinese Acad Sci, 19A Yuquan Rd,Shijingshan Zone, Beijing 100049, Peoples R China

3.Chinese Acad Sci, Wuhan Bot Garden, State Key Lab Plant Divers & Specialty Crops, 201 Jiufeng 1st Rd,Donghu New Technol Dev Zone, Wuhan 430074, Peoples R China

4.Xinjiang Acad Agr Sci, Turpan Inst Agr Sci, 845 Munaer Rd,Gaochang Zone, Turpan 838000, Peoples R China

5.North Carolina State Univ, Dept Hort Sci, 2721 Founders Dr, Raleigh, NC 27695 USA

6.Chinese Acad Sci, Inst Bot, Beijing Key Lab Grape Sci & Enol, 20 Nanxincun,Haiding Zone, Beijing 100093, Peoples R China

7.Chinese Acad Sci, Inst Bot, CAS Key Lab Plant Resources, 20 Nanxincun,Haiding Zone, Beijing 100093, Peoples R China

期刊名称：HORTICULTURE RESEARCH （ 影响因子：8.5； 五年影响因子：9.1 ）

ISSN： 2662-6810

年卷期： 2025 年 12 卷 4 期

页码：

收录情况： SCI

摘要： Although the significance of some plant WRKYs in response to cold stress have been identified, the molecular mechanisms of most WRKYs remain unclear in grapevine. In this study, we demonstrate that cold-induced expression of VaBAM3 in Vitis amurensis executes a beneficial role in enhancing resistance by the regulating starch decomposition. VaWRKY65 was identified as an upstream transcriptional activator of VaBAM3 through yeast one-hybrid library screening and validated to directly interact with the W-box region inside the VaBAM3 promoter. Transgenic Arabidopsis thaliana plants and grapevine roots overexpression VaWRKY65 exhibited improved cold tolerance along with higher BAM activity and soluble sugar levels, whereas opposite changes were observed in VaWRKY65 knockdown lines created by virus-induced gene silencing (VIGS) in grapevine plants and in the knockout wrky65 mutants generated by CRISPR/Cas9 technology in grapevine roots. The transcriptome data show that overexpression of VaWRKY65 led to significant alteration of a diverse set of stress-related genes at the transcriptional level. One of the genes, Peroxidase 36 (VaPOD36), was further verified as a direct target of VaWRKY65. Consistently, VaWRKY65-overexpressing plants had higher VaPOD36 transcript levels and POD activity but a reduced ROS level, while silencing VaWRKY65 results in contrary changes. Collectively, these results reveal that VaWRKY65 enhanced cold tolerance through modulating soluble sugars produced from starch breakdown and ROS scavenging.