您好，欢迎访问吉林省农业科学院 机构知识库！
吉林省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

A loss-of-function mutant allele of a glycosyl hydrolase gene has been co-opted for seed weight control during soybean domestication

文献类型： 外文期刊

作者： Wei, Siming 1 ; Yong, Bin 1 ; Jiang, Hongwei 4 ; An, Zhenghong 1 ; Wang, Yan 1 ; Li, Bingbing 1 ; Yang, Ce 1 ; Zhu, Weiwei 1 ; Chen, Qingshan 4 ; He, Chaoying 1 ;

作者机构： 1.Chinese Acad Sci, Inst Bot, State Key Lab Plant Divers & Specialty Crops, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China

2.China Natl Bot Garden, Beijing 100093, Peoples R China

3.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China

4.Northeast Agr Univ, Coll Agr, Harbin 150030, Peoples R China

5.Jilin Acad Agr Sci, Changchun 130033, Peoples R China

6.Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100101, Peoples R China

关键词： domestication; frameshift mutation; QTL; seed weight; soybean; to gain by loss

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：11.4； 五年影响因子：10.1 ）

ISSN： 1672-9072

年卷期： 2023 年 65 卷 11 期

页码：

收录情况： SCI

摘要： The resultant DNA from loss-of-function mutation can be recruited in biological evolution and development. Here, we present such a rare and potential case of "to gain by loss" as a neomorphic mutation during soybean domestication for increasing seed weight. Using a population derived from a chromosome segment substitution line of Glycine max (SN14) and Glycine soja (ZYD06), a quantitative trait locus (QTL) of 100-seed weight (qHSW) was mapped on chromosome 11, corresponding to a truncated & beta;-1, 3-glucosidase (& beta;Glu) gene. The novel gene hsw results from a 14-bp deletion, causing a frameshift mutation and a premature stop codon in the & beta;Glu. In contrast to HSW, the hsw completely lost & beta;Glu activity and function but acquired a novel function to promote cell expansion, thus increasing seed weight. Overexpressing hsw instead of HSW produced large soybean seeds, and surprisingly, truncating hsw via gene editing further increased the seed size. We further found that the core 21-aa peptide of hsw and its variants acted as a promoter of seed size. Transcriptomic variation in these transgenic soybean lines substantiated the integration hsw into cell and seed size control. Moreover, the hsw allele underwent selection and expansion during soybean domestication and improvement. Our work cloned a likely domesticated QTL controlling soybean seed weight, revealed a novel genetic variation and mechanism in soybean domestication, and provided new insight into crop domestication and breeding, and plant evolution.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：吉林省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台