您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Immune Response and Apoptosis-Related Pathways Induced by Aeromonas schubertii Infection of Hybrid Snakehead (Channa maculata female x Channa argus male)

文献类型： 外文期刊

作者： Liu, Chun 1 ; Ma, Jie 1 ; Zhang, Defeng 2 ; Li, Wei 1 ; Jiang, Biao 1 ; Qin, Zhendong 1 ; Su, Youlu 1 ; Lin, Li 1 ; Wang, Qi 1 ;

作者机构： 1.Zhongkai Univ Agr & Engn, Guangzhou Key Lab Aquat Anim Dis & Waterfowl Bree, Innovat Inst Anim Hlth Breeding, Coll Anim Sci & Technol, Guangzhou 510225, Peoples R China

2.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Aquat Anim Immune Technol Guangdong Prov, Guangzhou 510380, Peoples R China

3.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Fishery Drug Dev, Minist Agr, Guangzhou 510380, Peoples R China

关键词： hybrid snakehead (Channa maculata female x Channa argus male); Aeromonas schubertii; RNA-seq; immune response; apoptosis

期刊名称：PATHOGENS （ 影响因子：3.492； 五年影响因子：4.066 ）

ISSN：

年卷期： 2021 年 10 卷 8 期

页码：

收录情况： SCI

摘要： Aeromonas schubertii is the etiological pathogen of internal organ nodules in snakehead fish. Infections with A. schubertii produce a significant economic loss in aquaculture. Therefore, it is important to examine the immune mechanisms by which snakeheads defend against A. schubertii infection. In this study, we established a hybrid snakehead infection model by intraperitoneal injection of A. schubertii that produced internal organ nodules. The splenic immune response of infected fish was examined at the transcriptome level by Illumina-seq analysis. Results showed 14,796 differentially expressed genes (DEGs) following A. schubertii infection, including 4441 up-regulated unigenes and 10,355 down-regulated unigenes. KEGG analysis showed 2084 DEGs to be involved in 192 pathways, 14 of which were immune-related. Twelve DEGs were used to validate quantitative real-time PCR results with RNA-seq data. Time-course expression analysis of six genes demonstrated modulation of the snakehead immune response by A. schubertii. Furthermore, transcriptome analysis identified a substantial number of DEGs that were involved in the apoptosis signaling pathway. TUNEL analysis of infected spleens confirmed the presence of apoptotic cells. This study provided new information for a further understanding of the pathogenesis of A. schubertii in snakeheads, which can be used to prevent and possibly treat A. schubertii infections.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台