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Hif-1 alpha/Hsf1/Hsp70 signaling pathway regulates redox homeostasis and apoptosis in large yellow croaker (Larimichthys crocea) under environmental hypoxia

文献类型： 外文期刊

作者： Luo, Sheng-Yu 1 ; Wang, Jing-Qian 1 ; Liu, Cheng 1 ; Gao, Xin-Ming 1 ; Zhang, Yi-Bo 1 ; Ding, Jie 1 ; Hou, Cong-Cong 1 ; Zhu, Jun-Quan 1 ; Lou, Bao 2 ; Shen, Wei-Liang 3 ; Wu, Xiong-Fei 3 ; Zhang, Chun-Dan 1 ; Tang, Dao-Jun 1 ;

作者机构： 1.Ningbo Univ, Sch Marine Sci, Key Lab Appl Marine Biotechnol, Minist Educ, Ningbo 315211, Zhejiang, Peoples R China

2.Zhejiang Acad Agr Sci, Hangzhou 310021, Zhejiang, Peoples R China

3.Ningbo Acad Oceanol & Fishery, State Key Lab Large Yellow Croaker Breeding, Ningbo 315012, Zhejiang, Peoples R China

关键词： Hypoxia; Larimichthys crocea; Apoptosis; Redox homeostasis; Hif-1 alpha/Hsf1/Hsp70

期刊名称：ZOOLOGICAL RESEARCH （ 影响因子：6.975； 五年影响因子：4.811 ）

ISSN： 2095-8137

年卷期： 2021 年 42 卷 6 期

页码：

收录情况： SCI

摘要： Oxygen is an essential molecule for animal respiration, growth, and survival. Unlike in terrestrial environments, contamination and climate change have led to the frequent occurrence of hypoxia in aquatic environments, thus impacting aquatic animal survival. However, the adaptative mechanisms underlying fish responses to environmental hypoxia remain largely unknown. Here, we used large yellow croaker (Larimichthys crocea) and large yellow croaker fry (LYCF) cells to investigate the roles of the Hif-1 alpha/Hsf1/Hsp70 signaling pathway in the regulation of cellular redox homeostasis, and apoptosis. We confirmed that hypoxia induced the expression of Hif-1 alpha, Hsf1, and Hsp70 in vivo and in vitro. Genetic Hsp70 knockdown/overexpression indicated that Hsp70 was required for maintaining redox homeostasis and resisting oxidative stress in LYCF cells under hypoxic stress. Hsp70 inhibited caspase-dependent intrinsic apoptosis by maintaining normal mitochondrial membrane potential, enhancing Bcl-2 mRNA and protein expression, inhibiting Bax and caspase3 mRNA expression, and suppressing caspase-3 and caspase-9 activation. Hsp70 suppressed caspase-independent intrinsic apoptosis by inhibiting nuclear translocation of apoptosis-inducing factor (AIF) and disturbed extrinsic apoptosis by inactivating caspase-8. Genetic knockdown/overexpression of Hif-1 alpha and dual-luciferase reporter assay indicated that Hif-1 alpha activated the Hsf1 DNA promoter and enhanced Hsf1 mRNA transcription. Hsf1 enhanced Hsp70 mRNA transcription in a similar manner. In summary, the Hif-1 alpha/Hsf1/Hsp70 signaling pathway plays an important role in regulating redox homeostasis and anti-apoptosis in L. crocea under hypoxic stress.

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