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Genomic Characterization and Molecular Detection of Rehmannia Allexivirus Virus, a Novel Allexivirus Infecting Rehmannia glutinosa

文献类型： 外文期刊

作者： Qin, Yanhong 1 ; Lu, Shuhao 1 ; Wen, Yi 1 ; Li, Shaojian 1 ; Gao, Suxia 1 ; Liu, Yuxia 1 ; Li, Xuemeng 1 ; Yang, Jin 1 ; Wang, Fengli 1 ; Wang, Fei 1 ; Lu, Chuantao 1 ;

作者机构： 1.Henan Acad Agr Sci, Inst Plant Protect, Zhengzhou 450002, Peoples R China

关键词： Allexivirus; ReAV; genomic sequence; molecular variation; phylogenetic relationship

期刊名称：MICROORGANISMS （ 2022影响因子：4.5； 五年影响因子：4.8 ）

ISSN：

年卷期： 2024 年 12 卷 5 期

页码：

收录情况： SCI

摘要： Rehmannia glutinosa is one of the most important medicinal plants in China and is affected by viral diseases. In this study, a new virus tentatively named Rehmannia Allexivirus virus (ReAV) was identified through high-throughput sequencing, reverse-transcription polymerase chain reaction (RT-PCR), and Sanger sequencing. The complete genome length was 7297 nt and it contained five open reading frames (ORFs) encoding replicase, triple gene block 1(TGB1), TGB2, TGB3, and coat protein (CP). The replicase and CP presented nucleotide homology ranges of 59.9-65.2% and 47.5-55.5% between the nine ReAV isolates and the other 12 species of the genus Allexivirus. In the nine isolates, ReAV-20 and ReAV-31 isolates showed breakpoints in the replicase and CP regions, respectively. The other isolates shared 87.2-96.5% nt with the whole genome nucleotide identity. The phylogenetic tree showed that seven ReAV isolates based on replicase, CP, and whole genome sequences were clustered in the same branch and were related to the genus Allexivirus. The ReAV detection rates for 60 R. glutinosa samples were 73.3-81.7% through RT-PCR using primers targeting the replicase or CP genes. These results demonstrate that ReAV is the dominant virus in R. glutinosa. This study provides important evidence for understanding viruses infecting R. glutinosa and for establishing efficient strategies to prevent viral spread.

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