Comparative Analysis of Root Transcriptome of High-NUE Mutant and Wild-Type Barley under Low-Nitrogen Conditions
文献类型： 外文期刊
作者： Gao, Runhong 1 ; Zhou, Longhua 2 ; Guo, Guimei 2 ; Li, Yingbo 2 ; Chen, Zhiwei 2 ; Lu, Ruiju 2 ; Liu, Chenghong 2 ; Chen, Jianmin 1 ;
作者机构： 1.Yangzhou Univ, Coinnovat Ctr Modern Prod Technol Grain Crops Jian, Jiangsu Key Labs Crop Genet & Physiol & Plant Func, Minist Educ, Yangzhou 225009, Peoples R China
2.Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai 201106, Peoples R China
3.Shanghai Key Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China
关键词： barley (Hordeum vulgare L; ); low-N stress; root architecture; transcriptome; differentially expressed genes
期刊名称：AGRONOMY-BASEL （ 影响因子：3.7； 五年影响因子：4.0 ）
ISSN：
年卷期： 2023 年 13 卷 3 期
页码：
收录情况： SCI
摘要： Although nitrogen (N) deficiency greatly affects N absorption and metabolism in barley, the global transcriptomic changes in morphological and physiological adaptation to altered N availability remains largely unclear. We conducted a comparative transcriptome analysis of roots in A9-29 (low N tolerant line of barley) and Hua 30 (low N-sensitive variety of barley) under low N conditions to elucidate the responses and the underlying molecular mechanism. The results demonstrated that the root architecture was strongly influenced and that the root morphological indexes (total root length, total root area surface, and root volume) were remarkably promoted in A9-29 compared to Hua30 under low N stress. The transcriptome analysis of roots identified 1779 upregulated differentially expressed genes (DEGs) and 1487 downregulated DEGs specifically expressed in A9-29 under low N stress. Specific DEGs in A9-29 were largely enriched in energy metabolism, lipid metabolism, and the metabolism of other amino acids. In addition, transcription factor genes ERFs and IAA-related genes were specifically expressed in A9-29. To conclude, this study could provide a foundation for improving low N tolerance in barley.
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