Characterization of Fusarium solani Associated with Tobacco (Nicotiana tabacum) Root Rot in Henan, China
文献类型： 外文期刊
作者： Qiu, Rui 1 ; Li, Caihong 1 ; Zhang, Yingying 1 ; Li, Xiaojie 1 ; Li, Chengjun 1 ; Liu, Chang 1 ; Zhang, Mengdan 1 ; Bai, Jingke 1 ; Chen, Yuguo 1 ; Li, Fangfang 2 ; Li, Shujun 1 ;
作者机构： 1.Henan Acad Agr Sci, Tobacco Res Inst, Key Lab Green Preservat & Control Tobacco Dis & Pe, Xuchang 461000, Peoples R China
2.Henan Prov Tobacco Corp CNTC, Zhengzhou 450018, Henan, Peoples R China
关键词： cross-pathogenicity; genetic diversity; molecular analysis; morphological characteristics; Nectria haematococca
期刊名称：PLANT DISEASE （ 影响因子：4.4； 五年影响因子：4.8 ）
ISSN： 0191-2917
年卷期： 2024 年 108 卷 8 期
页码：
收录情况： SCI
摘要： The aim of this study was to characterize the Fusarium solani species complex (FSSC) population obtained from tobacco roots with root rot symptoms by morphological characteristics, molecular tests, and assessment of pathogenicity. Cultures isolated from roots were white to cream with sparse mycelium on potato dextrose agar, with colony growth of 21.5 +/- 0.5 to 29.5 +/- 0.5 mm after 3 days. Sporodochia were cream on carnation leaf agar (CLA) and Spezieller N & auml;hrstoffarmer agar (SNA), and macroconidia formed in sporodochia were 3 to 6 septate and straight to slightly curved, with wide central cells, a slightly short blunt apical cell, and a straight to almost cylindrical basal cell with a distinct foot shape, ranging in size from 20.92 to 64.37 x 3.91 to 6.57 mu m. Microconidia formed on CLA were reniform and fusiform, with 0 or 1 to occasionally 2 septa, that formed on long monophialidic conidiogenous cells, with a size range of 5.99 to 32.32 x 1.76 to 5.84 mu m. Globose to oval chlamydospores were smooth- to rough-walled, 6.5 to 13.3 +/- 0.37 mu m in diameter, and terminal or intercalary and occurred singly, in pairs, or occasionally in short chains on SNA. Molecular tests consisted of sequencing and phylogenetic analysis of the translation elongation factor-1 alpha (EF-1 alpha), RNA polymerase II largest subunit, and second largest subunit regions. All the obtained sequences revealed 98.14 to 100% identity to F. solani in both Fusarium ID and Fusarium MLST databases. Phylogenetic trees of the EF-1 alpha gene and concatenated three-locus data showed that isolates from tobacco in Henan grouped in the proposed group 5, which is nested within FSSC clade 3 (FSSC 5). Twenty-seven of the 28 isolates caused root rot in artificially inoculated tobacco seedlings, with a disease severity index ranging from 15.00 +/- 1.67 to 91.11 +/- 2.22. Cross-pathogenicity tests showed that three representative isolates were virulent to six species of Solanaceae and two species of Poaceae, with disease severity indexes ranging from 6.12 +/- 0.56 to 84.44 +/- 0.00, indicating that these isolates have a wide host range. The results may inform the control of tobacco root rot through improved crop rotations.
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