文献类型： 外文期刊

作者： Xia, Zhengjun ¹ ; Zhai, Hong ¹ ; Zhang, Yanfeng ² ; Wang, Yaying ¹ ; Wang, Lu ¹ ; Xu, Kun ¹ ; Wu, Hongyan ¹ ; Zhu, Jinglong ¹ ; Jiao, Shuang ³ ; Wan, Zhao ¹ ; Zhu, Xiaobin ¹ ; Gao, Yi ¹ ; Liu, Yingxiang ³ ; Fan, Rong ³ ; Wu, Shihao ⁴ ; Chen, Xin ⁴ ; Liu, Jinyu ¹ ; Yang, Jiayin ⁵ ; Song, Qijian ⁶ ; Tian, Zhixi ⁷ ;

作者机构： 1.Chinese Acad Sci, Innovat Acad Seed Design, Northeast Inst Geog & Agroecol, Key Lab Soybean Mol Design Breeding, Harbin 150081, Peoples R China

2.Hybrid Rapeseed Res Ctr Shaanxi Prov, Yangling 712100, Shaanxi, Peoples R China

3.Northeast Agr Univ, Coll Life Sci, Harbin 150030, Peoples R China

4.Jiangsu Acad Agr Sci, Inst Ind Crops, Nanjing 210014, Peoples R China

5.Jiangsu Acad Agr Sci, Huaiyin Inst Agr Sci Xuhuai Reg, Huaian 223001, Peoples R China

6.USDA ARS, Soybean Genome & Improvement Lab, Beltsville, MD 20705 USA

7.Chinese Acad Sci, Innovat Acad Seed Design, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China

关键词： QNE1; soybean; TCP; flowering time; vegetative period; maturity; Zhonghuang 13

期刊名称：SCIENCE CHINA-LIFE SCIENCES （ 影响因子：10.372； 五年影响因子：7.587 ）

ISSN： 1674-7305

年卷期：

页码：

收录情况： SCI

摘要： The soybean E1 gene is a major regulator that plays an important role in flowering time and maturity. However, it remains unclear how cultivars carrying the dominant E1 allele adapt to the higher latitudinal areas of northern China. We mapped the novel quantitative trait locus QNE1 (QTL near E1) for flowering time to the region proximal to E1 on chromosome 6 in two mapping populations. Positional cloning revealed Glyma.06G204300, encoding a TCP-type transcription factor, as a strong candidate gene for QNE1. Association analysis further confirmed that functional single nucleotide polymorphisms (SNPs) at nucleotides 686 and 1,063 in the coding region of Glyma.06G204300 were significantly associated with flowering time. The protein encoded by the candidate gene is localized primarily to the nucleus. Furthermore, soybean and Brassica napus plants overexpressing Glyma.06G204300 exhibited early flowering. We conclude that despite their similar effects on flowering time, QNE1 and E4 may control flowering time through different regulatory mechanisms, based on expression studies and weighted gene co-expression network analysis of flowering time-related genes. Deciphering the molecular basis of QNE1 control of flowering time enriches our knowledge of flowering gene networks in soybean and will facilitate breeding soybean cultivars with broader latitudinal adaptation.