Analysis of phagocytosis by mIgM plus lymphocytes depending on monoclonal antibodies against IgM of largemouth bass (Micropterus salmoides)
文献类型： 外文期刊
作者： Wu, Jing 1 ; Nie, Yifan 5 ; Ma, Yanping 2 ; Hao, Le 2 ; Liu, Zhenxing 2 ; Li, Yugu 1 ;
作者机构： 1.South China Agr Univ, Coll Vet Med, Guangzhou 510642, Peoples R China
2.Guangdong Acad Agr Sci, Inst Anim Hlth, Guangzhou 510640, Peoples R China
3.Key Lab Livestock Dis Prevent Guangdong Prov, Guangzhou 510640, Peoples R China
4.Minist Agr & Rural Affairs, Sci Observat & Expt Stn Vet Drugs & Diagnost Tech, Guangzhou 510640, Peoples R China
5.Zhongkai Univ Agr & Engn, Coll Anim Sci & Technol, Dept Aquaculture, Guangzhou 510225, Peoples R China
关键词： Largemouth bass; MAbs; IgM (+) lymphocytes; Phagocytosis; Fc gamma R
期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.622； 五年影响因子：4.799 ）
ISSN： 1050-4648
年卷期： 2022 年 123 卷
页码：
收录情况： SCI
摘要： The phagocytic actives of B cells in fish have been proven in recent years. In this study, five positive hybridomas secreting monoclonal antibodies (MAbs) against largemouth bass IgM were produced. Indirect immunofluores-cence assay (IFA) demonstrated that five MAbs could specifically recognize membrane-bound IgM (mIgM) molecule of largemouth bass. Indirect ELISA and Western blotting analysis showed that all the five MAbs had no cross-reactions with the other two teleost IgMs. Flow cytometry analysis (FCM) revealed that the percentages of largemouth bass mIgM+ lymphocytes in head kidney, peripheral blood and spleen were 51.66 +/- 0.608%, 16.5 +/- 1.235% and 42.92 +/- 1.091%, respectively. In addition, the phagocytosis rates of mIgM + lymphocytes ingesting Nocardia seriolae from head kidney, peripheral blood and spleen were calculated to be 5.413 +/- 0.274%, 16.6 +/- 0.289% and 26.3 +/- 0.296%, respectively. The qPCR results of sorted cells indicated that most inflammatory cytokines (IFN gamma, IL-1 beta, IL-2, IL-12 beta, IL-34, IL-10), chemokine (CXCL12), chemokines receptors (CXCR2, CXCR4) and genes (Fc gamma RIa, NCF1, CFL, ARP2/3, CD45, Syk, MARCKS) related to Fc gamma R-mediated phagocytic signaling pathway in phagocytic mIgM+ lymphocytes were up-regulated significantly (P < 0.05). Taken together, the results suggested that the MAb (MM06H) produced in this paper could be used as a tool to study mIgM+ lym-phocytes of largemouth bass, and Fc gamma R may participate in the phagocytosis of mIgM+ lymphocytes, which is helpful to further study the role of mIgM+ lymphocytes in innate immunity.
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