文献类型： 外文期刊

作者： Sun, Xueting ¹ ; Lu, Guangliang ¹ ; Sun, Ronghua ¹ ; Li, You ³ ; Sun, Shiwei ⁴ ; Gao, Lei ¹ ;

作者机构： 1.Key Lab Natl Forestry & Grassland Adm Ecol Landsca, Shanghai 200232, Peoples R China

2.Shanghai Acad Landscape Architecture Sci & Plannin, Shanghai Engn Res Ctr Landscaping Challenging Urba, Shanghai 200232, Peoples R China

3.Fujian Agr & Forestry Univ, Vector Borne Virus Res Ctr, Fujian Prov Key Lab Plant Virol, Fuzhou 350002, Fujian, Peoples R China

4.CATAS, Spice Beverage Res Inst, Hainan 571533, Wanning, Peoples R China

关键词： invasive species; ambrosia beetle; molecular tool; rapid; mitochondrial DNA

期刊名称：JOURNAL OF ECONOMIC ENTOMOLOGY （ 影响因子：2.2； 五年影响因子：2.3 ）

ISSN： 0022-0493

年卷期： 2023 年 116 卷 4 期

页码：

收录情况： SCI

摘要： Xylosandrus compactus (Eichhoff) (Coleoptera: Curculionidae, Scolytinae) is a worldwide invasive species that causes huge economic loss and environmental damage in many countries. Traditional morphological characteristics make it hard to identify scolytines due to their tiny size. Besides, the intercepted insect samples are incomplete, and the limitation of insect (larvae and pupae) morphology makes morphological identification more difficult. The majority of the damage is caused by adults and fungi that serve as nutrition for their larvae. They destroy plant trunks, branches, and twigs, affecting plant transport tissues in both weak and healthy plants. An accurate, efficient, and economical molecular identification technique for X. compactus not restricted by professional taxonomic knowledge is necessary. In the present study, a molecular identification tool based on the mitochondrial DNA gene, cytochrome C oxidase subunit I (COI) was developed. A species-specific COI (SS-COI) PCR assay was designed to identify X. compactus regardless of the developmental stage. Twelve scolytines commonly found in eastern China, namely Xylosandrus compactus, X. crassiusculus, X. discolor, X. germanus, X. borealis, X. amputates, X. eupatorii, X. mancus, Xyleborinus saxesenii, Euwallacea interjectus, E. fornicatus, and Acanthotomicus suncei, were included in the study. Additionally, specimens of X. compactus from 17 different areas in China, as well as a specimen collected from the United Stated, were also analyzed. Results demonstrated the accuracy and high efficiency of the assay, regardless of the developmental stage or the type of specimen. These features provide a good application prospect for fundamental departments and can be used to prevent the harmful consequences of the spread of X. compactus.