Identification and screening of fungicides against Piper nigrum basal Fusarium wilt disease in Hainan, China
文献类型： 外文期刊
作者： Liu, Shichao 1 ; Liu, Ruibing 1 ; Chu, Bo 3 ; Li, Zhuang 4 ; Meng, Qianqian 1 ; Gou, Yafeng 1 ; Xue, Chao 1 ; Tian, Tian 1 ; Chen, Pengyun 5 ; Wei, Fei 5 ; Wen, Siwei 1 ; Liu, Yanan 6 ; Sun, Shiwei 1 ; Gao, Shengfeng 1 ;
作者机构： 1.Chinese Acad Trop Agr Sci, Spice & Beverage Res Inst, Wanning, Peoples R China
2.Natl Ctr Important Trop Crops Engn & Technol Res, Wanning, Peoples R China
3.Henan Agr Univ, Coll Plant Protect, Zhengzhou, Peoples R China
4.Henan Univ Urban Construct, Coll Math & Phys, Pingdingshan, Peoples R China
5.Zhengzhou Univ, Sch Life Sci, Zhengzhou, Peoples R China
6.Yunnan Agr Univ, Coll Trop Crop Sci, Puer, Peoples R China
7.Chinese Acad Trop Agr Sci, Spice & Beverage Res Inst, Wanning 571533, Hainan, Peoples R China
关键词： fungicide; Fusarium solani; Fusarium wilt; pathogen identification; Piper nigrum
期刊名称：JOURNAL OF BASIC MICROBIOLOGY （ 影响因子：3.1； 五年影响因子：3.0 ）
ISSN： 0233-111X
年卷期： 2023 年
页码：
收录情况： SCI
摘要： Fusarium wilt has occurred in the main Piper nigrum cultivation regions, which seriously affects the yield and quality of P. nigrum. To identify the pathogen of this disease, the diseased roots were collected from a demonstration base in Hainan Province. The pathogen was obtained by tissue isolation method and confirmed by pathogenicity test. Based on the morphological observation, sequence analyses of TEF1-alpha nuclear gene, Fusarium solani was identified as the pathogen causing P. nigrum Fusarium wilt and induced symptoms on inoculated plants, including chlorosis, necrotic spots, wilt, drying, and root rot. The experiments for the antifungal activity showed that all the 11 fungicides selected in this study showed certain inhibitory effects on the colony growth of F. solani, where 2% kasugamycin AS, 45% prochloraz EW, 25 g center dot L-1 fludioxonil SC and 430 g center dot L-1 tebuconazole SC exhibited relative higher inhibitory effects with EC50 as 0.065, 0.205, 0.395, and 0.483 mg center dot L-1, respectively, and were selected to perform SEM analysis and test in seeds in vitro. The SEM analysis showed that kasugamycin, prochloraz, fludioxonil, and tebuconazole might have exerted their antifungal effect by damaging F. solani mycelia or microconidia. These preparations were applied as a seed coating of P. nigrum Reyin-1. The kasugamycin treatment was most effective in reducing the harmful impact of F. solani on the seed germination. These results presented herein provide useful guidance for the effective control of P. nigrum Fusarium wilt.
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