文献类型： 外文期刊

作者： Hongjuan, Han ¹ ; Fujian, Zhang ³ ; Guohua, Liang ¹ ; Quanhong, Yao ² ; Qiaoquan, Liu ¹ ;

作者机构： 1.Yangzhou Univ, Jiangsu Key Lab Crop Genom & Mol Breeding, Jiangsu Key Lab Crop Genet & Physiol, Key Lab Plant Funct Genom,Minist Educ, Yangzhou 225009, Peoples R China

2.Shanghai Acad Agr Sci, Agrobiotechnol Res Inst, Shanghai Key Lab Agr Genet & Breeding, 2901 Beidi Rd, Shanghai 201106, Peoples R China

3.Chongming Dist Agrotechnol Popularizat Ctr, Shanghai 202150, Peoples R China

关键词： Bacillus licheniformis; Glycine oxidase; Glyphosate resistance; Transgenic plants

期刊名称：BIOTECHNOLOGY LETTERS （ 影响因子：2.716； 五年影响因子：2.672 ）

ISSN： 0141-5492

年卷期：

页码：

收录情况： SCI

摘要： Objectives To find glycine oxidase genes that can be applied to the breeding of glyphosate resistant crops. Results The glycine oxidase (GO, EC 1.4.3.19) gene (GenBank No: KC831746) from Bacillus licheniformis (B. licheniformis) was chemically synthesized and transformed into glyphosate-sensitive Escherichia coli (E. coli). The GO gene was transformed into Arabidopsis and rice through Agrobacterium-mediated transformation. The test results confirmed that transgenic plants containing GO genes are more resistant to glyphosate than wild-type plants. On solid Murashige and Skoog (MS) (Murashige and Skoog1962 ) medium containing 200 mu M glyphosate, transgenic Arabidopsis thaliana grew normally, while wild-type plants were stunted and root growth was restricted. In a solution containing 500 mu M glyphosate, wild-type rice showed severe yellowing, while transgenic rice grew normally. In addition, when sprayed with 10 mM glyphosate solution, wild-type rice withered and died, while transgenic rice grew well. The function of GO gene in glyphosate resistance and the application value of GO gene in the cultivation of glyphosate-resistant crops is proved. Conclusions The glycine oxidase gene from B. licheniformis enhances the resistance of E. coli, Arabidopsis and rice to glyphosate.