您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Rapid identification and deployment of major genes for flowering time and awn traits in common wheat

文献类型： 外文期刊

作者： Wu, Jizhong 1 ; Qiao, Linyi 2 ; Liu, Ying 1 ; Fu, Bisheng 1 ; Nagarajan, Ragupathi 2 ; Rauf, Yahya 2 ; Jia, Haiyan 2 ; Yan, Liuling 2 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Germplasm Resources & Biotechnol, Nanjing, Jiangsu, Peoples R China

2.Oklahoma State Univ, Dept Plant & Soil Sci, Stillwater, OK 74078 USA

3.Shanxi Agr Univ, Coll Agron, Shanxi Key Lab Crop Genet & Mol Improvement, Taiyuan, Shanxi, Peoples R China

4.Nanjing Agr Univ, Appl Plant Genom Lab, Natl Key Lab Crop Genet & Germplasm Enhancement, Nanjing, Jiangsu, Peoples R China

关键词： Vrn-D1; flowering genes; Ali-A1; awn; common wheat

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：6.627； 五年影响因子：7.255 ）

ISSN： 1664-462X

年卷期： 2022 年 13 卷

页码：

收录情况： SCI

摘要： Molecular markers are developed to accelerate deployment of genes for desirable traits segregated in a bi-parental population of recombinant inbred lines (RILs) or doubled haplotype (DH) lines for mapping. However, it would be the most effective if such markers for multiple traits could be identified in an F-2 population. In this study, single nucleotide polymorphisms (SNP) chips were used to identify major genes for heading date and awn in an F-2 population without developing RILs or DH lines. The population was generated from a cross between a locally adapted spring wheat cultivar "Ningmaizi119" and a winter wheat cultivar "Tabasco" with a diverse genetic background. It was found that the dominant Vrn-D1 allele could make Ningmaizi119 flowered a few months earlier than Tabasco in the greenhouse and without vernalization. The observed effects of the allele were validated in F-3 populations. It was also found that the dominant Ali-A1 allele for awnless trait in Tabasco or the recessive ali-A1 allele for awn trait in Ningmaizi119 was segregated in the F-2 population. The allelic variation in the ALI-A1 gene relies not only on the DNA polymorphisms in the promoter but also on gene copy number, with one copy ali-A1 in Ningmaizi119 but two copies Ali-A1 in Tabasco based on RT-PCR results. According to wheat genome sequences, cultivar "Mattis" has two copies Ali-A1 and cultivar "Spelta" has four copies Ali-A in a chromosome that was uncharacterized (ChrUN), in addition to one copy on chromosome 5A. This study rapidly characterized the effects of the dominant Vrn-D1 allele and identified the haplotype of Ali-A1 in gene copy number in the F-2 segregation population of common wheat will accelerate their deployment in cycling lines in breeding.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台