Response signatures of intestinal microbiota and gene transcription of the pearl gentian grouper to Vibrio harveyi infection
文献类型： 外文期刊
作者： Wang, Fan 1 ; Xu, Jia 2 ; Wang, Zhiwen 1 ; Cao, Junming 4 ; Lu, Yishan 1 ;
作者机构： 1.Guangdong Ocean Univ, Shenzhen Inst, Guangdong Prov Engn Res Ctr Aquat Anim Hlth Assess, Shenzhen Publ Serv Platform Evaluat Marine Econ An, Shenzhen 518210, Peoples R China
2.Guangxi Acad Sci, Guangxi Acad Marine Sci, Guangxi Key Lab Marine Environm Sci, Nanning 530000, Guangxi, Peoples R China
3.Guangdong Ocean Univ, Coll Fishery, Zhanjiang 524088, Peoples R China
4.Guangdong Acad Agr Sci, Guangzhou 510640, Peoples R China
5.Guangdong Ocean Univ, Guangdong Prov Engn Res Ctr Aquat Anim Hlth Assess, Shenzhen Inst, Shenzhen 518210, Peoples R China
关键词： Transcriptome; Gut microbiotas; Vibrio harveyi; Grouper
期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.7； 五年影响因子：4.7 ）
ISSN： 1050-4648
年卷期： 2024 年 149 卷
页码：
收录情况： SCI
摘要： Vibrio harveyi causes high mortality and severely limits grouper culture. The gut microbiota is an important biological barrier against pathogen invasion. In this study, we investigated dynamic changes in the intestinal microbial community, gene transcription and immune responses signatures of pearl gentian grouper (Epinephelus fuscoguttatus male x Epinephelus lanceolatus female) at 0, 3 and 7 days (referred to as d0, d3 and d7 groups, respectively) after infection with V. harveyi. The results demonstrated that the d7 treatment reduced the gut microbial diversity and increased the proportion of Proteobacteria and Cyanobacteria. Notably, several putative pathogenic genera (Sphingomonas and Bacteroides) proliferated, while putative probiotic genera (Rhodococcus and Lactobacillus) reduced, and these changes in intestinal bacteria might be correlated to the alterations of host immunerelated molecules. The d3 and d7 treatments also altered the histomorphology and gene transcription profiles mainly associated with immune function in intestine, such as 'MAPK signaling pathway', 'Apoptosis' and 'Tolllike receptor (TLR) signaling pathway'. Furthermore, d3 group induced a homeostatic dysregulation of the antioxidant system, cytokines and TLR signaling, with a tendency to gradually return to a normal state in d7 group, along with the apoptosis process. The pathogenic infection suppressed the expression of JNK pathway and enhanced the ERK pathway. In conclusion, the dysbiosis of the intestinal bacterial communities caused by the immune changes that occurred during V. harveyi infection disrupted the intestine health in the pearl gentian grouper. These results provided a comprehensive understandings of the immune defense mechanisms in fish and valuable references to develop disease control strategies in grouper aquaculture.
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