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Genome-wide characterization of NBS-LRR family genes and expression analysis under powdery mildew stress in Lagenaria siceraria

文献类型： 外文期刊

作者： Wang, Jian 1 ; Yang, Chong 2 ; Wu, Xinyi 1 ; Wang, Ying 1 ; Wang, Baogen 1 ; Wu, Xiaohua 1 ; Lu, Zhongfu 1 ; Li, Guojing 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Vegetables, Hangzhou, Peoples R China

2.Guangdong Acad Sci, Inst Bioengn, Bioengn Res Lab, Guangzhou, Peoples R China

3.Zhejiang Acad Agr Sci, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou, Peoples R China

关键词： Bottle gourd; NBS-LRR family; Phylogenetic analysis; Powdery mildew; Expression analysis

期刊名称：PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY （ 影响因子：2.741； 五年影响因子：2.576 ）

ISSN： 0885-5765

年卷期： 2022 年 118 卷

页码：

收录情况： SCI

摘要： As the largest group of plant resistance genes, the nucleotide-binding site-leucine-rich repeat (NBS-LRR) genes play a critical role in plant disease defense system. The bottle gourd is a distinctive horticultural crop of the Cucurbitaceae family and powdery mildew is one of the major diseases jeopardizing crop production and quality. In this study, we have identified 84 NBS-LRR genes and divided into seven subfamilies. Phylogenetic tree and motif analysis grouped these genes into two major clusters with toll/interleukin-1 like receptor (TIR) domain or a coiled-coil domain. Subsequently, duplication events, motif sequence, orthologous gene cluster, protein-protein interaction, cis-elements were analyzed. 12 pairs of tandem duplicated genes and two pairs of segmental duplicated genes were detected in NBS-LRR family. 14 singleton genes and 62 functional cis-elements were found. The structure of 27 proteins were constructed by homology modeling and divided into five different types. 34 differentially expressed genes of NBS-LRR genes were found in PM-resistant and PM-susceptible line. Expression of NBS-LRR genes were validated by qRT-PCR under PM stress and gene Lsi04g015960 with powdery mildew8 (RPW8) domain was significantly up-regulated in PM-resistant variety. Our study provides fundamental insights of NBS-LRR family genes in bottle gourd and find a potential candidate gene Lsi04g015960 for PM tolerance breeding.

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