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Involvement of galectin-9 from koi carp (Cyprinus carpio) in the immune response against Aeromonas veronii infection

文献类型： 外文期刊

作者： Wang, Xiaowen 1 ; Liu, Lili 1 ; Zhang, Rong 1 ; Li, Huijuan 1 ; Zhu, Hua 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Key Lab Fishery Biotechnol, Beijing, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Fisheries Res Inst, Beijing, Peoples R China

3.Natl Freshwater Fisheries Engn Technol Res Ctr, Beijing, Peoples R China

4.Beijing Acad Agr & Forestry Sci, Fisheries Res Inst, Beijing 100068, Peoples R China

关键词： Koi carp(Cyprinuscarpio); Galectin-9; Immune response; Eukaryotic expression plasmid; Bacteriostasis function

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.622； 五年影响因子：4.799 ）

ISSN： 1050-4648

年卷期： 2022 年 129 卷

页码：

收录情况： SCI

摘要： Galectins are beta-galactoside sugar binding proteins which function as important pattern recognition receptors (PRRs) in innate immunity. Here, we identified a galectin-9 gene from koi carp (Cyprinus carpio), named kGal-9. The ORF of kGal-9 is 963 bp in length, which encodes a polypeptide of 320 amino acids without either signal peptide. The predicted molecular weight is 36.25 kDa, and the isoelectric point is 8.3. Multiple sequence alignment showed that the putative kGal-9 contains two carbohydrate recognition domains (CRD), which are conserved in Galectin-9s. The phylogenetic tree showed that kGal-9 clustered to Galectin-9s from other teleosts, and shared the highest identity of 87.5% with Qihe crucian (Carassius auratus). kGal-9 mRNA was abundant in head kidney, gills, and gut, but low in liver and muscle. Further, the expression level of kGal-9 in the head kidney and liver increased significantly after Aeromonas veronii (abbreviated A.v) infection. Unexpectedly, kGal-9 showed a remarkable downregulation in the spleen at various time points post A.v infection. Intramuscular injection of pckGal-9 not merely reduced the bacterial load of spleen tissue, but also improved the survival rate of koi carp post A.v challenge. Besides, administration of pckGal-9 stimulated the expression of several immuno-related genes including proinflammatory cytokines (IL-1 beta, IL-6), anti-inflammatory cytokine (IL-10), complement components (C4, C9), with fluctuation in spleen and head kidney. Taken together, the obtained results suggest that kGal-9 occupies an important role in innate immunity and defense against bacterial infection in koi carp.

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