文献类型： 外文期刊

作者： Huang, Mingjiao ¹ ; Gong, Peipan ¹ ; Yin, Cheng ¹ ; Yang, Jing ¹ ; Liu, Shaonan ¹ ; Fu, Buli ¹ ; Wei, Xuegao ¹ ; Liang, Jinjin ¹ ; Xue, Hu ¹ ; He, Chao ¹ ; Du, Tianhua ¹ ; Wang, Chao ¹ ; Ji, Yao ¹ ; Hu, Jinyu ¹ ; Zhang, Rong ¹ ; Belyakova, Natalia A. ⁵ ; Zhang, Youjun ¹ ; Yang, Xin ¹ ;

作者机构： 1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Dept Plant Protect, State Key Lab Vegetable Biobreeding, Beijing 100081, Peoples R China

2.Hunan Agr Univ, Coll Plant Protect, Changsha 410125, Peoples R China

3.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Key Lab Integrated Pest Management Trop Crops, Minist Agr & Rural Affairs, Haikou 571101, Peoples R China

4.Yangtze Univ, Coll Agr, Hubei Engn Technol Ctr Pest Forewarning & Manageme, Jingzhou 434025, Hubei, Peoples R China

5.Russian Acad Sci, All Russia Inst Plant Protect, Podbelskogo 3, St Petersburg 196608, Russia

关键词： Bemisia tabaci; P450s; Thiamethoxam-resistance; In vitro metabolism; Pest management

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：4.0； 五年影响因子：4.5 ）

ISSN： 0048-3575

年卷期： 2025 年 208 卷

页码：

收录情况： SCI

摘要： The whitefly Bemisia tabaci (Hemiptera: Gennadius) is a notorious and highly polyphagous agricultural pest that is well known for its ability to transmit a wide range of serious plant pathogenic viruses. The field populations of B. tabaci in some areas have developed resistance to thiamethoxam. We found that high expression of CYP6EM1 can enhance the resistance of B. tabaci to dinotefuran. It is unclear whether CYP6EM1 is involved in the resistance of B. tabaci to the same neonicotinoid pesticide, thiamethoxam. The results of the present study demonstrated that the expression of CYP6EM1 could be induced within 9 h after the exposure of B. tabaci adults to thiamethoxam. Molecular docking analyses, with a binding energy of-6.13 cal/mol, revealed a strong binding affinity between thiamethoxam and the CYP6EM1 protein, implying that CYP6EM1 may be involved in thiamethoxam resistance. Compared with that in the susceptible strain, the mRNA expression level of the CYP6EM1 gene was significantly greater in thiamethoxam-resistant strains (R#1, 9.93-fold, P = 0.0008; R#2, 40.43-fold, P = 0.0013; R#3, 27.40-fold, P = 0.0002; R#4, 21.63-fold, P = 0.0003 and R#5, 28.65-fold, P = 0.0006). Loss and gain of function studies in vivo were performed via RNA interference and transgenic expression in Drosophila melanogaster, and the results confirmed the role of CYP6EM1 in conferring such resistance. An in vitro metabolism assay revealed that CYP6EM1 directly metabolized 15.60 % of thiamethoxam. This study provides solid evidence for the critical role of CYP6EM1 in the metabolism of thiamethoxam, which contributes to resistance. Our work provides a deeper understanding of the mechanism underlying neonicotinoid resistance and contributes valuable insights for the sustainable management of global pests such as whiteflies.