Illustration of the variation in the content of flavanone rutinosides in various citrus germplasms from genetic and enzymatic perspectives
文献类型： 外文期刊
作者： Li, Wenyun 1 ; Li, Gu 1 ; Yuan, Ziyu 1 ; Li, Mingyue 1 ; Deng, Xiuxin 1 ; Tan, Meilian 3 ; Ma, Yuhua 2 ; Chen, Jiajing 1 ; Xu, Juan 1 ;
作者机构： 1.Huazhong Agr Univ, Coll Hort & Forestry, Key Lab Hort Plant Biol, Minist Educ, 1 Shizishan St, Wuhan 430070, Peoples R China
2.Guizhou Acad Agr Sci, Guizhou Fruit Inst, 1 Jinnong Rd, Guiyang 550006, Peoples R China
3.Chinese Acad Agr Sci, Oil Crops Res Inst, 2 Xudong Second Rd, Wuhan 430062, Peoples R China
期刊名称：HORTICULTURE RESEARCH （ 影响因子：7.291； 五年影响因子：7.487 ）
ISSN： 2662-6810
年卷期： 2022 年 9 卷
页码：
收录情况： SCI
摘要： In citrus, 1,6-rhamnosytransferase (1,6RhaT) and 1,2-rhamnosytransferase (1,2RhaT) catalyze flavanone-7-O-glucosides to form nonbitter flavanone rutinosides (FRs) and bitter flavanone neohesperidosides (FNs), respectively. As revealed in this study of fruit peels from 36 citrus accessions, FRs varied from undetectable levels in pummelo and kumquat to being the dominant flavonoids in sweet orange and loose-skin mandarins. Furthermore, a previously annotated full-length 1,6RhaT-like gene was identified as another 1,6RhaT-encoding gene by in vitro experiments. In total, 28 alleles of full-length 1,6RhaTs were isolated and classified into A, B and C types with only type A alleles encoding a functional protein. Coincidently, only the accessions that contained FRs harbored type A alleles, as was further verified in two F1 hybrid populations. Moreover, the inferior substrate conversion efficiency of 1,6RhaTs in comparison with that of 1,2RhaT in vitro might partly explain the lower proportions of FRs to total flavanone disaccharides in citrus hybrids harboring both functional rhamnosyltransferases. Our findings provide a better understanding of FR content variations among citrus and are meaningful for a mechanistic illustration of citrus flavonoid metabolism and fruit quality improvement practices.
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