Development of an Agrobacterium tumefaciens-mediated transformation system for Tilletia controversa Kuhn
文献类型： 外文期刊
作者： Du, Zhenzhen 1 ; Zong, Qianqian 1 ; Gao, Haifeng 3 ; Guo, Qingyuan 2 ; Liu, Taiguo 1 ; Chen, Wanquan 1 ; Gao, Li 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China
2.Xinjiang Agr Univ, Urumqi 830000, Xinjiang, Peoples R China
3.Xinjiang Acad Agr Sci, Inst Plant Protect, Key Lab Integrated Pest Management Crop North Wes, Minist Agr & Rural Affairs, Xinjiang 830091, Peoples R China
关键词： Dwarf bunt of wheat; Tilletia controversa Kuhn; Hygromycin B antibiotic; Agrobacterium tumefaciens-mediated; transformation (ATMT)
期刊名称：JOURNAL OF MICROBIOLOGICAL METHODS （ 影响因子：2.363； 五年影响因子：2.24 ）
ISSN： 0167-7012
年卷期： 2021 年 189 卷
页码：
收录情况： SCI
摘要： Dwarf bunt of wheat caused by Tilletia controversa Kuhn has been identified an international quarantine disease, which replace the grain material into millions of teliospores. Agrobacterium tumefaciens-mediated transformation (ATMT) system is a powerful tool for fungi transformation with significant advantages of simple operation, high efficiency, and genetic stability of transformants. In this study, we constructed ATMT system for T. controversa. All the transformants were tested using Acetosyringone (AS) concentration at 150 mu mol/l, hygromycin B at 25 mu g/ml, 1 x 10(6) T. controversa hypha cells/ml, A. tumefaciens with OD600 of 0.5 co-cultivation at 16 degrees C for 48 h and culture was incubated at 16 degrees C for 20 days. Using the ATMT method, we cultivated 8 generations of transformants on complete medium (CM) containing hygromycin B antibiotic and validated by PCR, which indicate that T-DNA had been successfully inserted into each of T. controversa transformants. In addition, thermal asymmetric interlaced PCR (TAIL-PCR) evaluated the Ti element inserts were at random sites in the fungal genome. Thus, ATMT approach is an efficient tool for insertional mutagenesis of T. controversa.
- 相关文献
作者其他论文 更多>>
-
Comparative analysis of stripe rust resistance in seedling stage and Yr gene incidence in spring and winter wheat from Xinjiang, China
作者：Lai, Hanlin;Li, Yue;Lai, Hanlin;Shen, Yuyang;Yang, Hong;Deng, Feifei;Chen, Li;Li, Guangkuo;Gao, Haifeng;Fernando, Dilantha W. G.;Ren, Chenrong;Lu, Yi;Wang, Huiqing;Sun, Na
关键词：wheat variety; disease resistance; molecular detection; Puccinia striiformis f. sp. tritici; Xinjiang
-
Exploring the potential of nanomaterials (NMs) as diagnostic tools and disease resistance for crop pathogens
作者：Jabran, Muhammad;Muhae-Ud-Din, Ghulam;Gao, Li;Gao, Li;Ali, Muhammad Amjad;Ali, Faizan;Muzammil, Saima;Zahoor, Adil;Hussain, Sarfaraz;Ijaz, Munazza;Ijaz, Munazza;Ijaz, Munazza
关键词：Nanomaterials; Disease resistance; Plant defense system; Plant pathogens; Disease identification; Eco-friendly
-
Analysis of microbial communities in wheat, alfalfa, and oat crops after Tilletia laevis Kühn infection
作者：Shen, Yuyang;Li, Guangkuo;Gao, Haifeng;Gao, Li;Delai, Chen;Liu, Taiguo;Chen, Wanquan;Gao, Li;Delai, Chen
关键词：fungal community; bacterial community; Tilletia laevis; RNA sequencing; soil properties; enzyme activities
-
Genetic Analysis Reveals Relationships Among Populations of Puccinia striiformis f. sp. tritici from Shaanxi-Gansu-Ningxia-Xinjiang of Northwestern and Sichuan-Yunnan of Southwestern China
作者：Zhang, Zhuoyue;Fu, Yanzi;Huang, Jie;Wang, Baotong;Li, Qiang;Peng, Yunliang;Zhou, Xinli;Gao, Haifeng
关键词：genetic diversity; northwestern and southwestern China; population structure; Puccinia striiformis f. sp. tritici
-
Life Table Parameters of the Tomato Leaf Miner Tuta absoluta (Lepidoptera: Gelechiidae) on Five Tomato Cultivars in China
作者：Yang, Hesen;Jiang, Hongbo;Yang, Hesen;Zhang, Guifen;Liu, Wanxue;Zhang, Yibo;Yang, Hesen;Zhang, Guifen;Liu, Wanxue;Zhang, Yibo;Zhang, Chi;Shen, Yuyang;Gao, Haifeng
关键词：life table; duration of preadult stage; lifetime fecundity; adult longevity
-
Screening of Endophytic Antagonistic Bacteria in Wheat and Evaluation of Biocontrol Potential against Wheat Stripe Rust
作者：Saimi, Ainisai;Zhang, Qiqi;Liu, Qi;Chen, Jing;Saimi, Ainisai;Zhang, Qiqi;Liu, Qi;Chen, Jing;Li, Guangkuo;Gao, Haifeng
关键词：wheat stripe rust; endophytic bacteria; antagonistic effect; biological control
-
First Report of Crown Rot Caused by Fusarium graminearum on Wheat in Xinjiang Uygur Autonomous Region, China
作者：Yang, Meixin;Li, Guangkuo;Gao, Haifeng;Yang, Meixin;Yi, Lishu;Liu, Taiguo;Zhang, Hao
关键词：cereals and grains; field crops; fungi; Fusarium crown rot; Fusarium graminearum; pathogen detection
