文献类型： 外文期刊

作者： Xiao, X. E. ¹ ; Wang, W. ¹ ; Crous, P. W. ³ ; Wang, H. K. ¹ ; Jiao, C. ¹ ; Huang, F. ⁵ ; Pu, Z. X. ⁷ ; Zhu, Z. R. ² ; Li, H. Y. ¹ ;

作者机构： 1.Zhejiang Univ, Key Lab Mol Biol Crop Pathogens & Insects, Minist Agr,Inst Biotechnol, Key Lab Biol Crop Pathogens & Insects Zhejiang Pr, 866 Yuhangtang Rd, Hangzhou 310058, Peoples R China

2.Zhejiang Univ, Hainan Inst, Sanya 572000, Peoples R China

3.Westerdijk Fungal Biodivers Inst, Uppsalalaan 8, NL-3584 CT Utrecht, Netherlands

4.Wageningen Univ & Res Ctr WUR, Lab Phytopathol, Droevendaalsesteeg 1, NL-6708 PB Wageningen, Netherlands

5.Guangdong Acad Agr Sci, Plant Protect Res Inst, Guangzhou 510640, Peoples R China

6.Guangdong Acad Agr Sci, Guangdong Prov Key Lab High Technol Plant Protect, Guangzhou 510640, Peoples R China

7.Citrus Res Inst Zhejiang Prov, Taizhou 318026, Peoples R China

关键词： Botryosphaeria cankers; distribution new taxa pathogenicity systematics

期刊名称：PERSOONIA （ 影响因子：11.658； 五年影响因子：11.388 ）

ISSN： 0031-5850

年卷期： 2021 年 47 卷

页码：

收录情况： SCI

摘要： Citrus is an important and widely cultivated fruit crop in South China. Although the species of fungal diseases of leaves and fruits have been extensively studied, the causal organisms of branch diseases remain poorly known in China. Species of Botryosphaeriaceae are known as important fungal pathogens causing branch diseases on citrus in the USA and Europe. To determine the diversity of Botryosphaeriaceae species associated with citrus branch diseases in China, surveys were conducted in the major citrus-producing areas from 2017 to 2020. Diseased tissues were collected from twigs, branches and trunks with a range of symptoms including cankers, cracking, dieback and gummosis. Based on morphological characteristics and phylogenetic comparison of the DNA sequences of the internal transcribed spacer region (ITS), the translation elongation factor 1-alpha gene (tef1), the beta-tubulin gene (tub2) and the DNA-directed RNA polymerase II second largest subunit (rpb2), 111 isolates from nine provinces were identified as 18 species of Botryosphaeriaceae, including Botryosphaeria dothidea, B. fabicerciana, Diplodia L. theobromae, Neodeightonia subglobosa, Neofusicoccum parvum, and six previously undescribed species, namely Do. citrimurcotticola, L. guilinensis, L. huangyanensis, L. linhaiensis, L. ponkanicola and Sphaeropsis linhaiensis spp. nov. Botryosphaeria dothidea (28.8 %) was the most abundant species, followed by L. pseudotheobromae (23.4 %), which was the most widely distributed species on citrus, occurring in six of the nine provinces sampled. Pathogenicity tests indicated that all 18 species of Botryosphaeriaceae obtained from diseased citrus tissues in this study were pathogenic to the tested Citrus reticulata shoots in vitro, while not all species are pathogenic to the tested Cocktail grapefruit (C. paradisi x C. reticulata) shoots in vivo. In addition, Lasiodiplodia was the most aggressive genus both in vitro and in vivo. This is the first study to identify Botryosphaeriaceae species related to citrus branch diseases in China and the results provide a theoretical basis for the implementation of prevention and control measures.