Transcriptomics reveals the mechanism of terbuthylazine-induced nephrotoxicity in chickens: Insights from AMPK/p53-mediated apoptosis perspective
文献类型： 外文期刊
作者： Yang, Qingwen 1 ; Ao, Da 2 ; Lv, Yongzhi 1 ; Liu, Xuesong 3 ;
作者机构： 1.Chongqing Three Gorges Vocat Coll, Dept Anim Sci & Technol, Lab Vet Pharmacol, Chongqing, Peoples R China
2.Changzhou Univ, Sch Pharm, Changzhou, Jiangsu, Peoples R China
3.Heilongjiang Acad Agr Sci, Branch Anim Husb & Vet, Lab Vet Pharmacol, Qiqihar, Peoples R China
关键词： Terbuthylazine; Apoptosis; AMPK/p53 pathway; Nephrotoxicity; Transcriptomics
期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：4.0； 五年影响因子：4.5 ）
ISSN： 0048-3575
年卷期： 2024 年 205 卷
页码：
收录情况： SCI
摘要： As a commonly used pesticide, the widespread use of terbuthylazine (TBA) may cause toxic effects in animals and human. However, the nephrotoxicity induced by TBA is unclear. Here, we explored the mechanism of TBAinduced nephrotoxicity through transcriptomics and molecular biology techniques in broilers. Pathologic analysis showed that TBA could cause renal cell vacuolation and fibrosis in broilers. Additionally, transcriptomic analysis showed that TBA can cause significant changes in the expression of some apoptosis-related genes, and GO and KEGG analysis also found that TBA can significantly change the functions of apoptosis pathway and AMPK signaling pathway in kidney. Subsequently, the protein expression levels of Bax, Bak-1, FADD, and cleaved Caspase-3/Caspase-3 were elevated significantly and the number of TUNEL-positive cells was increased markedly in kidney under TBA exposure. Meanwhile, we also found that TBA could activate AMPK/p53 pathway, as evidenced by the upregulated levels of AMPK alpha 1 phosphorylation and protein expression of p53. Therefore, our results suggested that TBA could induce apoptosis via AMPK/p53 pathway in kidney. These findings identified the nephrotoxic mechanism of TBA through transcriptomics, providing a new insight into TBA toxicology.
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