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Transcriptome analysis reveals the effect of grafting on gossypol biosynthesis and gland formation in cotton

文献类型： 外文期刊

作者： Ye, Kun 1 ; Teng, Teng 1 ; Yang, Teng 1 ; Zhao, Degang 1 ; Zhao, Yichen 1 ;

作者机构： 1.Guizhou Univ, Coll Tea Sci, Key Lab Plant Resources Conservat & Germplasm Inno, Coll Life Sci,Minist Educ, Guiyang 550025, Peoples R China

2.Guizhou Acad Agr Sci, Plant Conservat Technol Ctr, Guizhou Key Lab Agr Biotechnol, Guiyang 550006, Peoples R China

关键词： Cotton; Grafting; Pigment gland density; Gossypol content; Gossypol synthesis related genes; Transcriptome analysis; Differentially expressed genes; Real-time PCR analysis

期刊名称：BMC PLANT BIOLOGY （ 影响因子：5.3； 五年影响因子：5.9 ）

ISSN： 1471-2229

年卷期： 2023 年 23 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundGossypol is a unique secondary metabolite and sesquiterpene in cotton, which is mainly synthesized in the root system of cotton and exhibits many biological activities. Previous research found that grafting affected the density of pigment glands and the gossypol content in cotton.ResultsThis study performed a transcriptome analysis on cotton rootstocks and scions of four grafting methods. The gene expression of mutual grafting and self-grafting was compared to explore the potential genes involved in gossypol biosynthesis. A total of six differentially expressed enzymes were found in the main pathway of gossypol synthesis-sesquiterpene and triterpene biosynthesis (map00909): lupeol synthase (LUP1, EC:5.4.99.41), beta-amyrin synthase (LUP2, EC:5.4.99.39), squalene monooxygenase (SQLE, EC:1.14.14.17), squalene synthase (FDFT1, EC:2.5.1.21), (-)-germacrene D synthase (GERD, EC:4.2.3.75), ( +)-delta-cadinene synthase (CADS, EC:4.2.3.13). By comparing the results of the gossypol content and the density of the pigment gland, we speculated that these six enzymes might affect the biosynthesis of gossypol. It was verified by qRT-PCR analysis that grafting could influence gene expression of scion and stock. After suppressing the expression of the LUP1, FDFT1, and CAD genes by VIGS technology, the gossypol content in plants was significantly down-regulated.ConclusionsThese results indicate the potential molecular mechanism of gossypol synthesis during the grafting process and provide a theoretical foundation for further research on gossypol biosynthesis.

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