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Comparative Proteomic Analysis of Flammulina filiformis Reveals Substrate-Specific Enzymatic Strategies for Lignocellulose Degradation

文献类型： 外文期刊

作者： Li, Weihang 1 ; Han, Jiandong 1 ; Xie, Hongyan 1 ; Sun, Yi 2 ; Li, Feng 2 ; Gong, Zhiyuan 1 ; Zou, Yajie 2 ;

作者机构： 1.Shandong Acad Agr Sci, Inst Agr Resources & Environm, State Key Lab Nutrient Use & Management, Minist Agr & Rural,Key Lab Wastes Matrix Utilizat, Jinan 250100, Peoples R China

2.Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, State Key Lab Efficient Utilizat Arable Land China, Beijing 100081, Peoples R China

关键词： Flammulina filiformis; lignocellulose degradation; secretory proteomics

期刊名称：HORTICULTURAE （ 影响因子：3.0； 五年影响因子：3.2 ）

ISSN：

年卷期： 2025 年 11 卷 8 期

页码：

收录情况： SCI

摘要： Flammulina filiformis, one of the most delicious and commercially important mushrooms, demonstrates remarkable adaptability to diverse agricultural wastes. However, it is unclear how different substrates affect the degradation of lignocellulosic biomass and the production of lignocellulolytic enzymes in F. filiformis. In this study, label-free comparative proteomic analysis of F. filiformis cultivated on sugarcane bagasse, cotton seed shells, corn cobs, and glucose substrates was conducted to identify degradation mechanism across various substrates. Label-free quantitative proteomics identified 1104 proteins. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis of protein expression differences were predominantly enriched in energy metabolism and carbohydrate metabolic pathways. Detailed characterization of carbohydrate-active enzymes among the identified proteins revealed glucanase (GH7, A0A067NSK0) as the key enzyme. F. filiformis secreted higher levels of cellulases and hemicellulases on sugarcane bagasse substrate. In the cotton seed shells substrate, multiple cellulases functioned collaboratively, while in the corn cobs substrate, glucanase predominated among the cellulases. These findings reveal the enzymatic strategies and metabolic flexibility of F. filiformis in lignocellulose utilization, providing novel insights for metabolic engineering applications in biotechnology. The study establishes a theoretical foundation for optimizing biomass conversion and developing innovative substrates using targeted enzyme systems.

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