Using Magnetic Molecularly Imprinted Polymer Technology for Determination of Fish Serum Glucose Levels
文献类型： 外文期刊
作者： Yao, Boxuan 1 ; Gu, Long 1 ; Huang, Li 1 ; Li, Ruichun 1 ; Fan, Ze 1 ; Chen, Zhongxiang 1 ; Qin, Dongli 1 ; Gao, Lei 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Heilongjiang River Fisheries Res Inst, Harbin 150070, Peoples R China
2.Dalian Ocean Univ, Coll Food Sci & Engn, Dalian 116023, Peoples R China
3.Minist Agr & Rural Affairs, Inspection & Testing Ctr Fishery Environm & Aquat, Supervis, Harbin 150070, Peoples R China
4.Minist Agr & Rural Affairs, Key Lab Control Qual & Safety Aquat Prod, Beijing 100141, Peoples R China
关键词： glucose; serum; magnetic solid-phase extraction; selective extraction; derivatization
期刊名称：POLYMERS （ 2023影响因子：4.7； 五年影响因子：4.9 ）
年卷期： 2024 年 16 卷 11 期
收录情况： SCI
摘要： In this study, a highly efficient magnetic molecularly imprinted polymer nanocomposite material was prepared using multi-walled carbon nanotubes as carriers. The characterization of the obtained nanocomposite material was conducted using Fourier transform infrared spectroscopy, a vibrating sample magnetometer, a thermogravimetric analyzer, a scanning electron microscope, and a transmission electron microscope. The adsorption properties of the nanocomposite material were evaluated through adsorption experiments, including static adsorption, dynamic adsorption, and selective recognition studies. The prepared nanocomposite material, serving as a selective adsorbent, was applied in magnetic solid-phase extraction. Subsequently, the derivatized samples were analyzed for glucose in fish serum using liquid chromatography-tandem mass spectrometry. Under optimal conditions, the detection limit was 0.30 ng/mL, the quantitation limit was 0.99 ng/mL, satisfactory spiked recovery rates were obtained, and the relative standard deviation was less than 1.1%. Using 2-deoxy-D-ribose as the template molecule and a structural analog of glucose allowed us to eliminate the potential template leakage in qualitative and quantitative analyses, effectively avoiding the issues of false positives and potential quantitative errors, compared to traditional methods. A method for detecting glucose levels in fish serum based on molecularly imprinted polymer technology has been successfully developed to determine the stress and health levels of fish.
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