文献类型： 外文期刊

作者： Zhao, LM ¹ ; Dong, YS ² ; Li, B ² ; Hao, S ³ ; Wang, KJ; Li, XH;

作者机构： 1.NE Normal Univ, Inst Genet & Cytol, Changchun 130024, Peoples R China

2.NE Normal Univ, Inst Genet & Cytol, Changchun 130024, Peoples R China; Jilin Acad Agr Sci, Natl Ctr Plant Transgen Res & Comercializat, Gongzhuling 136100, Peoples R China; Jilin Univ, Sch Life Sci, Changchun 130021, Peoples R China; Chinese Acad Agr Sci, Inst Crop Germplasm Resources, Beijing 100081, Peoples R China

3.NE Normal Univ, Inst Genet & Cytol, Changchun 130024, Peoples R China; Jilin Acad Agr Sci, Natl Ctr Plant Transgen Res & Comercializat, Gongzhuling 136100, Peoples R China; Jilin Univ, Sch Life Sci, Changchun 130021, Peoples R China; Chinese Acad Agr Sci, Inst Crop Germplasm Resources, Beijing 100081, Peoples R

关键词： Core Collection;genetic diversity;wild soybean (Glycine Soja);sampling strategies

期刊名称：CHINESE SCIENCE BULLETIN （ 影响因子：1.649； 五年影响因子：1.738 ）

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收录情况： SCI

摘要： With more than 6000 accessions collected from across China, the Chinese National Crop Gene Bank (CNCGB) holds the largest collection of annual wild soybean (Glycine Soja) gerrnplasm in the world. To facilitate the management and utilization of these germplasm collections, it is important to establish a Core Collection. This study compares five sampling strategies, namely random, constant, proportional, logarithmic and genetic diversity-based, to establish a Core Collection for the anneal wild soybean gerniplasm. Among the strategies evaluated, the genetic diversity-based was found to be the simplest and most efficient. Using hierarchical classification and cluster analysis, in the genetic diversity diversity-based strategy, 652 accessions, accounting for 10.65 percent of the total 6172 accessions, were selected out to represent the total accessions. The established Core Collection has the following features: (i) the 21 descriptors observed in the entire collection were compared by the Core Collection, all 18 quality characters of entire collection were preserved by core collection, and coincidence rate of average was 98.4 percent; (ii) the variant of 13 descriptors of the two collections was very similar, with the coincidence index being 0.96; (iii) the coincidence rate of genetic diversity between the two collections was 81.38 percent DNA aides; (iv) the geographic distribution pattern of core collection was the same as the entire collection; (v) molecular marker analysis by 20 SSE primer pairs on 299 accessions showed that the Core Collection covered 83.64 percent of the entire collection. It thus is concluded that the established Core Collection is representative and will be a valuable entry point for better evaluation and more efficient utilization of the genetic resources available in the annual wild soybean germplasm bank.