文献类型： 外文期刊

作者： Zhao, Jiajia ¹ ; Wang, Zeng ² ; Yin, Jiaming ¹ ; Wei, Ying ² ; Li, Mingshan ² ; Ma, Zhongzheng ³ ; Yin, Meizhen ⁴ ; Dong, Min ¹ ; Shen, Jie ¹ ; Yan, Shuo ¹ ;

作者机构： 1.China Agr Univ, Sanya Inst, Sanya 572025, Peoples R China

2.China Agr Univ, Coll Plant Protect, Dept Plant Biosecur, State Key Lab Agr & Forestry Biosecur, Beijing 100193, Peoples R China

3.Beijing Acad Agr & Forestry Sci, Inst Plant Protect, Beijing 100097, Peoples R China

4.Beijing Univ Chem Technol, State Key Lab Chem Resource Engn, Beijing Lab Biomed Mat, Beijing 100029, Peoples R China

关键词： Co-delivery system; Drug adjuvant; Gene nanocarrier; Multicomponent RNA nano-biopesticide; Increased insecticide susceptibility

期刊名称：JOURNAL OF NANOBIOTECHNOLOGY （ 影响因子：12.6； 五年影响因子：12.3 ）

ISSN：

年卷期： 2025 年 23 卷 1 期

页码：

收录情况： SCI

摘要： High resistance of bean flower thrips (BFT, Megalurothrips usitatus) has led to the unscientific application of insecticides to cause famous "toxic cowpea" incidents in China. Nuclear factor erythroid 2-related factor 2 (Nrf2) plays an important role in inducing antioxidant responses and drug detoxification. Therefore, the detoxification genes may be suppressed to control insecticide resistance via Nrf2. Herein, we demonstrated that the expression of most detoxification genes and enzyme activity were remarkably suppressed via nrf2 RNAi. Subsequently, a novel hydrophilic-lipophilic diblock polymer (HLDP) was developed to co-assemble with dsNrf2 and sulfoxaflor (SUL) into nanoscale SUL/HLDP/dsNrf2 complex (221.52 nm). Excitingly, the SUL/HLDP/dsNrf2 complex exhibited excellent leaf adhesion performance, with the smaller contact angle, reduced surface tension, amplified contact area, improved retention, and enhanced plant uptake. Meanwhile, theSUL/HLDP/dsNrf2 displayed high delivery efficiency in vitro and in vivo, and its insecticidal activity against BFTs was significantly higher than SUL. Furthermore, the required doses of SUL/HLDP/dsNrf2 to achieve similar insecticidal activity were 50.14% and 58.42% of SUL via immersion and oral feeding, respectively. Overall, this study elucidated the regulatory role of nrf2 in the detoxification and metabolism of BFTs and developed a self-assembled multicomponent RNA nano-biopesticide to increase the susceptibility of BFTs to insecticides.