文献类型： 外文期刊

作者： Ma, Zhongzheng ¹ ; Zhang, Yunhui ¹ ; Li, Mingshan ¹ ; Chao, Zijian ¹ ; Du, Xiangge ¹ ; Yan, Shuo ¹ ; Shen, Jie ¹ ;

作者机构： 1.China Agr Univ, Coll Plant Protect, Dept Plant Biosecur, Beijing 100193, Peoples R China

2.China Agr Univ, Coll Plant Protect, MOA Key Lab Pest Monitoring & Green Management, Beijing 100193, Peoples R China

3.Beijing Acad Agr & Forestry Sci, Inst Plant Protect, Beijing 100097, Peoples R China

关键词： HpRNA expression system; Nanocarrier; Nanocarrier; hpRNA pesticide; RNA interference; RNA pesticide

期刊名称：JOURNAL OF PEST SCIENCE （ 影响因子：5.742； 五年影响因子：5.562 ）

ISSN： 1612-4758

年卷期：

页码：

收录情况： SCI

摘要： There are two main limitations for sprayable RNA pesticide development: delivery efficiency and synthetic cost of double-stranded RNA (dsRNA). We previously constructed a nanocarrier-based transdermal dsRNA delivery system and a novel bacteria-based hairpin RNA (hpRNA) expression system to solve these challenges. Herein, as a subsequent exploration of RNA pesticide (sprayable ds/hpRNA for pest control), we performed a greenhouse application of bacteria-expressed and nanocarrier-delivered RNA pesticide on green peach aphid. The nanoparticle SPc could combine and deliver dsRNA across the aphid cuticle and V-type proton ATPase subunits d (ATP-d) and G (ATP-G) were selected as the potential RNA interference (RNAi) targets. Our plasmid-Escherichia coli system simultaneously expressing ATP-d and ATP-G hairpin RNAs (hpRNAs) was constructed for mass production of hpRNA. The expressed hpRNA was mixed with SPc and detergent to form RNA formulation, which showed a certain insecticidal activity through the spray application in the greenhouse. Total control efficacy of our RNA pesticide could reach 61% on 3 d and maintained at 50% until the sixth day. To our knowledge, our study is the first attempt to apply the bacteria-expressed and nanocarrier-delivered RNA pesticides for pest control in the greenhouse trial, which is beneficial for promoting the development of RNA pesticides.