文献类型： 外文期刊
作者： Fan, Xing 1 ; Wei, Jinzi 1 ; Guo, Yu 1 ; Ma, Juan 1 ; Qi, Meiyu 2 ; Huang, He 1 ; Zheng, Peng 1 ; Jiang, Wenjie 1 ; Yao, Yuchang 1 ;
作者机构： 1.Northeast Agr Univ, Coll Anim Sci & Technol, Harbin 150038, Peoples R China
2.Heilongjiang Acad Agr Sci, Inst Anim Husb, Harbin 150086, Peoples R China
关键词： LPS; endometrial receptivity; embryo implantation; STAT1; sheep
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）
ISSN： 1661-6596
年卷期： 2024 年 25 卷 24 期
页码：
收录情况： SCI
摘要： Uterine infections reduce ruminant reproductive efficiency. Reproductive dysfunction caused by infusion of Gram-negative bacteria is characterized by the failure of embryo implantation and reduced conception rates. Lipopolysaccharide (LPS), a major component of the outer membrane of Gram-negative bacteria, is highly abortogenic. In this study, the effects of LPS infusion on the endometrial receptivity of sheep were studied during three critical periods of embryo implantation. The results showed that LPS infusion on d12, d16, and d20 of pregnancy in vivo interfered with the expression of prostaglandins (PGs) and affected the expression of adhesion-related factors (ITGB1/3/5, SPP1), key implantation genes (HOXA10, HOXA11 and LIF), and progestational elongation genes (ISG15, RSAD2 and CXCL10) during embryo implantation. In addition, after LPS infusion on d12, d16, and d20, the phosphorylation level of STAT1 significantly decreased and the protein expression level of IRF9 significantly increased on d12, suggesting that LPS infusion in sheep impairs endometrial receptivity through the JAK2/STAT1 pathway. Sheep endometrial epithelial cells were treated with 17 beta-estrogen, progesterone, and/or interferon-tau in vitro to mimic the receptivity of the endometrium during early pregnancy for validation. LPS and the p-STAT1 inhibitor fludarabine were both added to the model, which resulted in reduced p-STAT1 protein expression, significant inhibition of PGE2/PGF2 alpha, and significant suppression of the expression of key embryo implantation genes. Collectively, these results indicate that LPS infusion in sheep on d12, d16, and d20 impairs endometrial receptivity through the JAK2/STAT1 pathway, which is responsible for LPS-associated pregnancy failure.
- 相关文献
作者其他论文 更多>>
-
LPS Regulates Endometrial Immune Homeostasis and Receptivity Through the TLR4/ERK Pathway in Sheep
作者：Wei, Jinzi;Fan, Xing;Zang, Xiaorui;Guo, Yu;Jiang, Wenjie;Yao, Yuchang;Qi, Meiyu;Han, Hongbing
关键词：embryo implantation; inflammation; endometrial receptivity; TLR4
-
miR-192-2 Regulates the Proliferation and Apoptosis of Ovarian Granulosa Cells by Targeting IGFBP2 in Zhedong White Geese
作者：Wang, Size;Zeng, Chuicheng;Pan, Yue;Zang, Zhengyu;Huang, He;Zhang, Yuanliang;Yue, Shan;Zhao, Xiuhua
关键词：granulosa cells; miR-192-2; IGFBP2; cell proliferation; apoptosis; goose
-
Study on the effects of anthocyanins on the growth performance, immunity, and meat quality of ZI geese
作者：Huang, He;Wang, Size;Zeng, Chuicheng;Zang, Zhengyu;Pan, Yue;Wang, Junying;Zheng, Peng;Zhang, Yuanliang;Yue, Shan;Zhao, Xiuhua
关键词：Zi goose; Anthocyanin; Feed efficiency; Immunity; Meat quality
-
Integrative analysis of metabolites and microbial diversity revealed metabolic mechanism of coarse feeding tolerance in Songliao Black sows during gestation
作者：Zhao, Jinbo;Zhao, Wenjiang;Dong, Jiaqiang;Zhang, Hong;Yang, Kun;Gao, Shengyue;Feng, Wanyu;Song, Yan;Qi, Meiyu;He, Xinmiao
关键词：dietary fiber; metabolites; microbial community composition; Songliao sow; coarse feeding tolerance
-
TLR4 Overexpression Aggravates Bacterial Lipopolysaccharide-Induced Apoptosis via Excessive Autophagy and NF-& kappa;B/MAPK Signaling in Transgenic Mammal Models
作者：Wang, Sutian;Huang, Qiuyan;Yang, Yecheng;Meng, Fanming;Li, Jianhao;Luo, Chenglong;Zhang, Kunli;Song, Xuting;Lu, Qi;Zhao, Duowei;Yao, Yuchang;Lin, Sen;Deng, Shoulong;Deng, Shoulong;Qi, Meiyu;Lian, Zhengxing
关键词：Toll-like receptor 4; transgenic animal model; autophagy; apoptosis; inflammation; oxidative stress
-
Comparisons of adipogenesis- and lipid metabolism-related gene expression levels in muscle, adipose tissue and liver from Wagyu-cross and Holstein steers
作者：Liu, Li;Qi, Meiyu;Wang, Liang;Li, Zhongqiu;Zhao, Xiuhua;Zhao, Xiaochuan;Xu, Shanshan;Zhang, Haifeng;Wang, Genlin;Liu, Di;Sun, Fang;Cao, Peili;Zhang, Lupei;Shao, Guang;Ding, Liyan;Chai, Jinbao;Yue, Mengmeng
关键词：
-
Overexpression of Toll-Like Receptor 4 Affects Autophagy, Oxidative Stress, and Inflammatory Responses in Monocytes of Transgenic Sheep
作者：Wang, Sutian;Song, Xuting;Jiao, Peixin;Yao, Yuchang;Wang, Sutian;Zhang, Kunli;Deng, Shoulong;Qi, Meiyu;Lian, Zhengxing
关键词：TLR4; autophagy; oxidative stress; inflammatory responses; transgenic animal model; p38 MAPK
