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Transcriptome analysis of easy- and hard-to-root tea plants uncovers roles for CsGH3.2 and CsGH3.3 in adventitious root formation

文献类型： 外文期刊

作者： Chen, Rong 1 ; Zhao, Degang 2 ; Huang, Xiaozhen 1 ;

作者机构： 1.Guizhou Univ, Coll Tea Sci, Guiyang 550025, Peoples R China

2.Guizhou Univ, Coll Life Sci, Minist Educ, Key Lab Plant Resources Conservat & Germplasm Inn, Guiyang 550025, Peoples R China

3.Guizhou Acad Agr Sci, Guiyang 550006, Peoples R China

关键词： Camellia sinensis; Adventitious root formation; Rooting; Transcriptome analysis; Tea plants

期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.726； 五年影响因子：2.859 ）

ISSN： 0167-6857

年卷期： 2022 年 150 卷 2 期

页码：

收录情况： SCI

摘要： Poor adventitious root (AR) formation poses a major constraint to the propagation of tea cuttings. The present study identified two tea cultivars with different rooting behavior: Camellia sinensis cv. Taixuan 0310 (TX), with strong and rapid rooting; and C. sinensis cv. Qianmei 601 (QM), with poor and slow rooting. To elucidate the molecular events underlying AR formation in tea cuttings, transcriptomic analysis was performed during early root formation and root elongation. Differentially expressed genes (DEGs) between TX and QM were identified. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed that phenylpropanoid biosynthesis, plant hormone signal transduction, plant MAPK signaling and plant-pathogen interaction, all play important roles in AR information in C. sinensis. Among the DEGs, two GRETCHEN HAGEN3 (GH3) genes, CsGH3.2 and CsGH3.3, were isolated and characterized. Quantitative real-time PCR (qRT-PCR) and molecular analysis suggest that CsGH3.2 and CsGH3.3 may have specific functions in different organs. Moreover, overexpression of CsGH3.2 and CsGH3.3 inhibited root growth and development in transgenic rice plant. These results give new insights into the possible role of CsGH3.2 and CsGH3.3 in adventitious rooting, and also provide an interesting starting point to discover new genes involved in AR formation in tea cuttings. Key message Transcriptomic analysis revealed the molecular events underlying adventitious root formation in tea cuttings, and facilitated the discovery of genes involved in this process. CsGH3.2 and CsGH3.3 appear to function in adventitious rooting in tea cuttings.

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