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Transcriptome and Proteome Reveal Heat Shock Promotes Haploid Induction Rate via Activating ABA Signal Transduction in Watermelon

文献类型： 外文期刊

作者： Gong, Shiqi 1 ; Tang, Bingqian 1 ; Dai, Yujuan 1 ; Sun, Xiangyu 1 ; Song, Huijuan 1 ; Xiong, Cheng 1 ; Zou, Tian 2 ; Sun, Longjun 3 ; Liu, Guang 4 ; Yang, Hongbo 1 ; Zhu, Shengxiu 5 ; Dai, Sihui 1 ; Sun, Xiaowu 1 ;

作者机构： 1.Hunan Agr Univ, Coll Hort, Changsha 410128, Peoples R China

2.Hunan Biol & Electromech Polytech, Coll Plant Technol, Changsha 410127, Peoples R China

3.Hunan Xuefeng Seeds Co Ltd, Shaoyang 422000, Hunan, Peoples R China

4.Jiangsu Acad Agr Sci, Inst Vegetable Res, Nanjing 210014, Jiangsu, Peoples R China

5.Xinjiang Western Oasis Ecol Dev Co Ltd, Karamay 834000, Peoples R China

关键词： watermelon; haploid; heat shock; transcriptome; proteome

期刊名称：AGRONOMY-BASEL （ 影响因子：3.4； 五年影响因子：3.8 ）

ISSN：

年卷期： 2025 年 15 卷 5 期

页码：

收录情况： SCI

摘要： Haploid breeding technology has advantages in terms of saving time and reducing labor intensity and costs. However, the low induction rate limits the application of this technology. Previous researchers found that heat shock can increase the rate of Embryo-like structures (ELSs) induction. However, molecular mechanisms underlying heat-shocked haploid induction remain poorly understood. In the current study, unfertilized ovules of watermelon were subjected to heat shock for 0-5 days and conducted transcriptomics sequencing and DIA-based proteomics sequencing. Results indicated that, in contrast to the non-heat-shock condition, the expression level of protein phosphatase 2C (PP2C), a negative regulator in abscisic acid (ABA) signal transduction pathway, was repressed, and the expression level of Sucrose-non-fermenting 1-related protein kinases (SnRK2) was activated. The activated SnRK2s are enabled to promote the accumulation of storage substances in ovules. Through analysis, the expression of many genes involved in the biosynthesis of unsaturated fatty acids and amino acids has indeed been upregulated. In conclusion, our findings demonstrate that heat shock promotes the accumulation of storage substances in unfertilized ovules by activating the signal transduction process of ABA, which correspondingly increases ELSs induction rate.

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