您好，欢迎访问中国热带农业科学院 机构知识库！
中国热带农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Control of Aedes mosquito populations using recombinant microalgae expressing short hairpin RNAs and their effect on plankton

文献类型： 外文期刊

作者： Fei, Xiaowen 1 ; Xiao, Sha 1 ; Huang, Xiaodan 1 ; Li, Zhijie 1 ; Li, Xinghan 2 ; He, Changhao 1 ; Li, Yajun 2 ; Zhang, Xiuxia 2 ; Deng, Xiaodong 2 ;

作者机构： 1.Hainan Med Coll, Dept Biochem & Mol Biol, Haikou, Peoples R China

2.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou, Peoples R China

3.Hainan Inst Trop Agr Resources, Key Lab Biol & Genet Resources Trop Crops Hainan P, Haikou, Peoples R China

4.Hainan Prov Key Lab Funct Components Res & Utiliza, Haikou, Peoples R China

5.Zhanjiang Expt Stn, CATAS, Zhanjiang, Peoples R China

期刊名称：PLOS NEGLECTED TROPICAL DISEASES （ 影响因子：3.8； 五年影响因子：4.1 ）

ISSN： 1935-2735

年卷期： 2023 年 17 卷 1 期

页码：

收录情况： SCI

摘要： Author summaryA new low-cost strategy was developed to control vector mosquitoes safely. Chlorella was genetically modified to produce siRNA targeting the mosquito chsa gene, inhibiting gene expression after the larval stage. The inhibition prevented metamorphosis with high larval mortality in both Aedes aegypti and Aedes albopictus. The recombinant Chlorella performed well in the simulated field trials but impacted plankton populations in the released water, which indicates that the technology requires further optimization for field use. This approach is promising for biorational mosquito control to prevent mosquito-borne diseases. New biocontrol strategies are urgently needed to combat vector-borne infectious diseases. This study presents a low-cost method to produce a potential mosquito insecticide that utilizes the microalgae released into suburban water sources to control mosquito populations. Chlorella microalgae are ubiquitous in local waters, which were chosen as the host for genetic transfection. This species facilitated the recombinant algae to adapt to the prevailing environmental conditions with rapid growth and high relative abundance. The procedure involved microalgae RNAi-based insecticides developed using short hairpin RNAs targeting the Aedes aegypti chitin synthase A (chsa) gene in Chlorella. These insecticides effectively silenced the chsa gene, inhibiting Aedes metamorphosis in the laboratory and simulated-field trials. This study explored the impact of recombinant microalgae on the phytoplankton and zooplankton in suburban waters. High-throughput sequencing revealed that rapid reproduction of recombinant Chlorella indirectly caused the disappearance of some phytoplankton and reduced the protozoan species. This study demonstrated that a recombinant microalgae-based insecticide could effectively reduce the population of Aedes mosquitoes in the laboratory and simulated field trials. However, the impact of this technology on the environment and ecology requires further investigation.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国热带农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台