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Utilization of recombinant Chlorella expressing dsRNA for protection against grouper nervous necrosis virus

文献类型： 外文期刊

作者： Wei, Mengzhi 1 ; Fei, Xiaowen 3 ; Hong, Peng 1 ; Liu, Shihang 1 ; Deng, Xiaodong 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 571101, Peoples R China

2.Hainan Inst Trop Agr Resources, Key Lab Biol & Genet Resources Trop Crops Hainan P, Haikou 571101, Peoples R China

3.Hainan Med Coll, Dept Biochem & Mol Biol, Haikou, Peoples R China

4.Hainan Prov Key Lab Funct Components Res & Utiliza, Haikou, Peoples R China

5.CATAS, Zhanjiang Expt Stn, Zhanjiang 524013, Peoples R China

关键词： Chlorella sorokiniana; Nervous necrosis virus; Pearl gentian grouper; IFN innate immunity

期刊名称：AQUACULTURE REPORTS （ 影响因子：3.2； 五年影响因子：3.5 ）

ISSN： 2352-5134

年卷期： 2024 年 38 卷

页码：

收录情况： SCI

摘要： In mammals and fish, dsRNA can induce the type I interferon (IFN-I), leading to the expression of antiviral and immune-related genes. The use of microalgae expressing dsRNA as an oral delivery system to activate innate immunity in fish has not been documented. In this study, wild Chlorella sorokiniana was transformed with NNV (nervous necrosis virus) RdRp and GIV (grouper iridovirus) ORF086R dsRNA expression vectors. The recombinant Chlorella strains effectively protected pearl gentian groupers ( Epinephelus fuscoguttatus female female x Epinephelus lanceolatus male) male ) against NNV and reduced viral titers. The survival rates of the NNV RdRp and GIV ORF086R dsRNA treatment groups increased by 32 % and 38 %, respectively, compared with that of the control group. These findings indicate that recombinant Chlorella has the potential to be used as an oral delivery strategy for protection against NNV disease in groupers.

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