Root predominant overexpression of iaaM and CKX genes promotes root initiation and biomass production in citrus
文献类型： 外文期刊
作者： Tang, Dan 1 ; Li, Yanjun 2 ; Zhai, Longmei 2 ; Li, Wei 2 ; Kumar, Rahul 2 ; Yer, Huseyin 2 ; Duan, Hui 4 ; Cheng, Baoping 5 ; Deng, Ziniu 1 ; Li, Yi 2 ;
作者机构： 1.Hunan Agr Univ, Coll Hort, Changsha, Hunan, Peoples R China
2.Univ Connecticut, Dept Plant Sci & Landscape Architecture, Storrs, CT 06279 USA
3.China Agr Univ, Coll Hort, Beijing 100193, Peoples R China
4.USDA ARS, US Natl Arboretum Floral & Nursery Plants Res Unit, Beltsville Agr Res Ctr BARC West, Beltsville, MD 20705 USA
5.Guangdong Acad Agr Sci, Plant Protect Res Inst, Guangdong Prov Key Lab High Technol Plant Protect, Guangzhou 510640, Peoples R China
关键词： iaaM gene; CKX gene; Root-predominant expression; Root initiation and growth; Root biomass; Citrus
期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：3.0； 五年影响因子：3.0 ）
ISSN： 0167-6857
年卷期： 2023 年
页码：
收录情况： SCI
摘要： Promoting initiation and biomass production of roots is significant for plant-based industries including using roots as bioreactors. Two citrus genotypes, Carrizo and US-897, were used as model plants to test the effects of root-predominantly overexpressed the iaaM (indoleacetic acid-tryptophan monooxygenase) gene and a CKX (a cytokinin oxidase/dehydrogenase) gene. The iaaM transgenic lines exhibited markedly faster root initiation, more root numbers, and higher root biomass compared to their wild-type counterparts. The transgenic iaaM + CKX plants also exhibited similar phenotypes, albeit to a lesser extent than the iaaM plants. Molecular analysis revealed an auxin-responsive CsGH3.1 gene was up-regulated in the iaaM roots and iaaM + CKX roots, and a cytokinin-responsive gene CsARR5 gene was down-regulated in the iaaM + CKX roots. Our results demonstrate that root predominant overexpression of the iaaM or both the iaaM and CKX genes drastically enhances the initiation, growth and biomass production of roots. These results provide additional support that manipulation of auxin and cytokinin levels in roots via transgenic or gene-editing technologies may benefit production of high-value secondary metabolites using roots as bioreactors and also improve rooting of recalcitrant plant species. Key messageRoot-predominant overexpression of an auxin synthetic gene and a cytokinin degradation gene in citrus enhance initiation and biomass production of roots.
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