文献类型： 外文期刊

作者： Xia, Xuefeng ¹ ; Song, Shiying ¹ ; Zhang, Shuangyu ¹ ; Wang, Wei ¹ ; Zhou, Junming ¹ ; Fan, Baochao ¹ ; Li, Li ¹ ; Dong, Hailong ³ ; Luo, Chuping ⁴ ; Li, Bin ¹ ; Zhang, Xuehan ¹ ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Vet Med, Key Lab Vet Biol Engn & Technol, Minist Agr Minist, Nanjing 210014, Jiangsu, Peoples R China

2.Jiangsu Univ, Sch Food & Biol Engn, 301 Xuefu Rd, Zhenjiang 212013, Jiangsu, Peoples R China

3.Acad Anim Sci, Xizang Agr & Anim Husb Coll, Linzhi 860000, Peoples R China

4.Huaiyin Inst Technol, Jiangsu Prov Key Construct Lab Probiot Preparat, Huaian 223003, Peoples R China

5.Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225000, Jiangsu, Peoples R China

6.Minist Sci & Technol, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base, Nanjing 210014, Jiangsu, Peoples R China

7.Jiangsu Acad Agr Sci, 50 Zhongling St, Nanjing 210014, Jiangsu, Peoples R China

关键词： Antimicrobial peptides; Combination use; Food safety; Gram-negative bacteria; Membrane integrity; Vegetables

期刊名称：INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY （ 影响因子：5.4； 五年影响因子：5.5 ）

ISSN： 0168-1605

年卷期： 2023 年 386 卷

页码：

收录情况： SCI

摘要： Escherichia coli O157:H7 is a pathogen that commonly causes foodborne illness and represents a health hazard to consumers. The combined use of synergistic antimicrobial peptides (AMPs) is a promising way to improve the microbiological safety of foods. In this study, we detected the synergistic interactions between thanatin and BF -15a3 to reduce their usage and obtain more efficient antibacterial activity. The minimal inhibitory concentra-tions (MICs) of thanatin and BF-15a3 against 49 E. coli O157:H7 strains were ranged from 2 to 8 mu g/mL and 4-32 mu g/mL, showed a general inhibitory effect on E. coli O157:H7 strains, respectively, even multidrug-resistant strains. Their fractional inhibitory concentration index (FICI) was 0.375, which suggested that their combina-tion presented synergistic antibacterial effect against E. coli O157:H7. The killing kinetic curves indicated that the 0.25 x MIC combination had equivalent bactericidal effects to 1 x MIC thanatin or BF-15a3. When AMP combinations were used to treat eukaryotic cells to evaluate the hemolytic characteristics against rabbit eryth-rocytes and cytotoxicity against human embryonic kidney 293T (HEK-293T) cells and intestinal porcine enter-ocyte J2 (IPEC-J2) cells, no magnified adverse effects were observed, exhibiting higher specificity to bacteria and lower toxicity to eukaryotic cells. Compared with bacteriostasis of thanatin or BF-15a3 alone, the proportion of membrane-damaged bacteria treated with the synergetic combination did not appear a significant rise, inter-estingly the Zeta potential of them greatly decreased and their cell membrane permeability significantly increased. Besides, more release of ions and cytoplasm were detected, confirming a more severe loss of mem-brane integrity. These results suggested that the synergistic action mode of thanatin and BF-15a3 is likely attributed to damage aggravation to E. coli membrane. When applying in fresh-cut lettuce and cucumber, their combination allowed for 2.5 log CFU/piece reductions of E. coli O157:H7 in 24 h. In conclusion, the combination of thanatin and BF-15a3 showed excellent synthetic efficacy to kill E. coli O157:H7 in vitro under lower MICs than single use of them.