Carrageenan oligosaccharides and associated carrageenan-degrading bacteria induce intestinal inflammation in germ-free mice
文献类型： 外文期刊
作者： Yin, Yeshi 1 ; Li, Miaomiao 2 ; Gu, Weizhong 3 ; Zeng, Benhua 4 ; Liu, Wei 1 ; Zhu, Liying 1 ; Pi, Xionge 1 ; Primerano, 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, Inst Food Res, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310021, Peoples R China
2.Ocean Univ China, Key Lab Marine Drugs, Minist Educ, Shandong Prov Key Lab Glycosci & Glycotechnol, Qingdao 266100, Peoples R China
3.Zhejiang Univ, Childrens Hosp, Sch Med, Dept Gastroenterol, Hangzhou 310057, Peoples R China
4.Third Mil Med Univ, Coll Basic Med Sci, Dept Lab Anim Sci, Chongqing 400038, Peoples R China
5.Marshall Univ, Joan C Edwards Sch Med, Dept Biomed Sci, Huntington, WV 25701 USA
6.Hunan Univ Sci & Engn, 130 Yang Zitang Rd, Yongzhou 425199, Hunan, Peoples R China
关键词： Carrageenans; Carrageenan oligosaccharides; Oligosaccharide degrading bacteria; Intestinal inflammatory; Germ-free mouse
期刊名称：JOURNAL OF GENETICS AND GENOMICS （ 影响因子：4.275； 五年影响因子：5.223 ）
ISSN： 1673-8527
年卷期： 2021 年 48 卷 9 期
页码：
收录情况： SCI
摘要： Carrageenans (CGNs) are widely used in foods and pharmaceuticals although their safety remains controversial. To investigate the effects of CGNs and CGN- degrading bacteria in the human colon, we screened for CGN degradation by human fecal microbiota, and for inflammatory response to CGNs and/or CGN-degrading bacteria in germ free mice. Thin-layer chromatography indicated that high molecular weight (MW) CGNs (>= 100 kDa) remained undegraded in the presence of human fecal microbiota, whereas low MW CGNs, i.e., kappa-carrageenan oligosaccharides (KCO,similar to 4.5 kDa) were degraded when exposed to seven of eight human fecal samples, although sulfate groups were not removed during degradation. Bacteroides xylanisolvens and Escherichia coli isolates from fecal samples apparently degraded KCO synergistically, with B. xylanisolvens serving as the primary degrader. Combined treatment of KCO with KCO-degrading bacteria led to greater pro-inflammatory effects in the colon and rectum of germ-free mice than either KCO or bacteria alone. Similarly, p-p38-, CD3-, and CD79a-positive immune cells were more abundant in combined treatment group mice than in either single treatment group. Our study shows that KCO-degrading bacteria and the low MW products of KCO can promote proinflammatory effects in mice, and represent two key markers for evaluating CGN safety in foods or medicines. Copyright (C) 2021, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Limited and Science Press. All rights reserved.
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