Molecular cloning, expression, and functional analyses of plasmanylethanolamine desaturase gene of Takifugu rubripes
文献类型： 外文期刊
作者： Ji, Hong-Long 1 ; Liu, Chang 2 ; Zhang, Jing-Jing 2 ; Lin, Lei 2 ; Yang, Qi 2 ; Yang, Yu 2 ; Dong, Cai-Chao 2 ; He, Yang-bin 2 ; Shao, Chang-wei 2 ;
作者机构： 1.Zhejiang Ocean Univ, Coll Fisheries, Zhoushan 316022, Peoples R China
2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, State Key Lab Mariculture Biobreeding & Sustainabl, Qingdao 266071, Shandong, Peoples R China
3.Qingdao Marine Sci & Technol Ctr, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Shandong, Peoples R China
关键词： Plasmalogen; Tmem189; Expression
期刊名称：GENE （ 影响因子：2.4； 五年影响因子：2.8 ）
ISSN： 0378-1119
年卷期： 2025 年 953 卷
页码：
收录情况： SCI
摘要： The aging population has led to a significant increase in neurodegenerative diseases, particularly Alzheimer's disease (AD), which adversely affects the quality of life and longevity of the elderly. Abnormal plasmalogen metabolism plays a crucial role in the pathogenesis of AD. This study focused on tmem189, a key gene involved in plasmalogen synthesis. We successfully cloned and characterized the open reading frame (ORF) of tmem189 in Takifugu rubripes, revealing that it encodes a protein consisting of 275 amino acids. Notably, tmem189 expression was found to be higher in brain compared to other tissues. We transfected a GFP-tagged eukaryotic expression vector into 293 T cells, confirming successful expression of tmem189 with increased expression levels. Additionally, liquid chromatography-mass spectrometry (LC-MS) analysis demonstrated that tmem189 promotes plasmalogen synthesis in the transfected 293T cells. Our findings suggest that tmem189 could serve as a potential target for the treatment of neurodegenerative diseases, providing new insights into the promotion of plasmalogen synthesis.
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