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Identification of potential roles of cathepsin L1 in the response to alkaline treatment in Macrobrachium nipponense

文献类型： 外文期刊

作者： Xu, Mingjia 1 ; Zhang, Wenyi 2 ; Xiong, Yiwei 2 ; Fu, Hongtuo 1 ; Qiao, Hui 1 ; Jiang, Sufei 1 ; Jin, Shubo 1 ;

作者机构： 1.Nanjing Agr Univ, Wuxi Fisheries Coll, Wuxi 214081, Peoples R China

2.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Key Lab Freshwater Fisheries & Germplasm Resources, Minist Agr & Rural Affairs, Wuxi 214081, Peoples R China

关键词： Macrobrachium nipponense; Alkaline treatment; Cathepsin L1; QPCR; RNAi

期刊名称：AQUACULTURE INTERNATIONAL （ 影响因子：2.4； 五年影响因子：2.7 ）

ISSN： 0967-6120

年卷期： 2025 年 33 卷 6 期

页码：

收录情况： SCI

摘要： Cathepsin L (CTSL) is an important cysteine protease, which plays an important role in the process of environmental stress in aquatic animals. Previous study reported that CTSL of Macrobrachium nipponense (MnCTSL1) may be involved in the alkaline stress response. To further elucidate its potential function under alkali treatment in M. nipponense, this study employed integrated approaches including quantitative real-time PCR (qPCR), in situ hybridization (ISH), and RNA interference (RNAi) to systematically investigate the response of MnCTSL1 to alkaline treatment. qPCR analysis revealed ubiquitous MnCTSL1 expression across all tested tissues of M. nipponense, with the expression level in the hepatopancreas significantly surpassing those in other tissues in both sexes. Significant upregulation of MnCTSL1 expression was observed in hepatopancreas and gills under the alkalinity of both 5 mmol/L and 10 mmol/L, suggesting its involvement in alkaline stress response. ISH results showed that MnCTSL1 mRNA signals were detected in the basement membrane of hepatopancreas, and in the hemolymph vessels and membranes of gills in both sexes. After knockdown of MnCTSL1 expression using RNAi, the cumulative mortality of prawns in the dsCTSL1-injected group was 2.50- and 2.52-fold higher than that in the dsGFP-injected group at 5 mmol/L and 10 mmol/L alkalinity, respectively, suggesting that MnCTSL1 plays an important role in regulation of alkaline acclimatization of M. nipponense. These findings demonstrate that MnCTSL1 serves as a crucial regulator of alkaline adaptation in this commercially important crustacean species.

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